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Chemiluminescent detection of DNA: application for DNA sequencing and hybridization
S Beck1, T O'Keeffe, J M Coull
1MilliGen/Biosearch, Division of Millipore, Burlington, MA 01803.
Nucleic Acids Research
|July 11, 1989
Abstract:
A non-radioactive DNA detection chemistry is described and its application is shown for DNA hybridization and standard dideoxy DNA sequencing. The method employes a biotin-streptavidin system which binds an enzyme specifically to a target DNA and upon exposure to substrate, the enzyme catalyzes a chemiluminescent reaction. The image is captured within seconds by a Polaroid or X-ray film. The method is capable of detecting DNA in the hundred attomol range.