Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Construction of a 42 base pair double stranded DNA microcircle.

M Wolters¹, B Wittig

¹Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, FRG.

Nucleic Acids Research

|July 11, 1989

Summary

Researchers created the smallest double-stranded DNA (dsDNA) microcircles yet, measuring 42 base pairs. This breakthrough enables high-resolution structural analysis of DNA microcircles using techniques like X-ray crystallography.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The effects of hypothetical behavioral interventions on the 13-year incidence of overweight/obesity in children and adolescents.

The international journal of behavioral nutrition and physical activity·2023

Same author

Dietary calcium intake and adiposity in children and adolescents: Cross-sectional and longitudinal results from IDEFICS/I.Family cohort.

Nutrition, metabolism, and cardiovascular diseases : NMCD·2019

Same author

Potentially modifiable determinants of malnutrition in older adults: A systematic review.

Clinical nutrition (Edinburgh, Scotland)·2019

Same author

Immunotherapeutic maintenance treatment with toll-like receptor 9 agonist lefitolimod in patients with extensive-stage small-cell lung cancer: results from the exploratory, controlled, randomized, international phase II IMPULSE study.

Annals of oncology : official journal of the European Society for Medical Oncology·2018

Same author

The TLR9 agonist MGN1703 triggers a potent type I interferon response in the sigmoid colon.

Mucosal immunology·2017

Same author

Blood fatty acid composition in relation to allergy in children aged 2-9 years: results from the European IDEFICS study.

European journal of clinical nutrition·2016

Area of Science:

Molecular Biology
Biochemistry
Structural Biology

Background:

Established DNA circularization methods have limitations in producing very small DNA circles.
The precise structural analysis of DNA requires well-defined, stable DNA constructs.

Purpose of the Study:

To develop a novel procedure for constructing extremely small double-stranded DNA (dsDNA) microcircles.
To overcome limitations of existing circularization techniques for creating minimal DNA structures.
To generate sufficient quantities of these microcircles for advanced structural studies.

Main Methods:

Utilized two synthetic oligonucleotides designed to form dumbbell-shaped structures.
Performed two sequential ligation reactions to achieve DNA microcircle formation.

Related Experiment Videos

Characterized the resulting dsDNA microcircles, confirming their size and structure.

Main Results:

Successfully constructed dsDNA microcircles of 42 base pairs, the smallest reported to date.
The procedure overcomes natural limitations associated with DNA circularization.
Produced microcircles in quantities suitable for high-resolution structural analyses.

Conclusions:

The described method provides a robust way to synthesize ultra-small dsDNA microcircles.
These 42 bp dsDNA microcircles are valuable tools for structural biology.
This advancement facilitates high-resolution structural analyses like X-ray crystallography and NMR spectroscopy.