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A Morphometric and Cellular Analysis Method for the Murine Mandibular Condyle
Published on: January 11, 2018
Quantitative Proteomic Analysis of Rat Condylar Chondrocytes during Postnatal Development
Insights
This study identified 137 differentially expressed proteins in rat mandibular condylar cartilage (MCC) chondrocytes during early postnatal development. These proteins are crucial for growth, cellular processes, and catalytic activity, offering insights into MCC development.
Area of Science:
- Biochemistry
- Molecular Biology
- Developmental Biology
Background:
- The mandibular condylar cartilage (MCC) is critical for jaw development and function.
- Understanding protein expression changes during early postnatal growth is essential for comprehending MCC development.
Purpose of the Study:
- To investigate differentially expressed proteins in rat MCC chondrocytes during initial mastication for short postnatal periods.
- To identify key proteins involved in the early growth and development of MCC.
Main Methods:
- Protein samples were extracted from rat MCC chondrocytes at postnatal days 1, 7, 14, and 28.
- Isobaric tags for relative and absolute quantification (iTRAQ) labeling, 2D nano-HPLC, and MALDI-TOF/TOF mass spectrometry were employed.
- Bioinformatics analysis using PANTHER and MASCOT software identified differentially expressed proteins and their functions.
Main Results:
- 137 differentially expressed proteins were identified in rat MCC chondrocytes.
- Proteins were involved in metabolic processes, cellular processes, biological regulation, developmental processes, and catalytic activity.
- Significant protein expression changes were observed during the tooth eruption growth stage.
Conclusions:
- This research provides a comprehensive view of the molecular mechanisms governing early postnatal rat MCC growth and development at the protein level.
- The findings highlight the dynamic proteomic changes in MCC chondrocytes during development.
Objective:
To investigate differentially expressed proteins in rat mandibular condylar cartilage (MCC) chondrocytes caused by initial mastication for short postnatal periods.
Methods:
Four groups of protein samples were extracted from primary cultured rat MCC chondrocytes, harvested from eigthy postnatal SD rats aged 1,7,14 and 28 days, with twenty in each group. Total proteins were labelled with isobaric tags for relative and absolute quantification (iTRAQ) reagents. Two-dimensional nano-high-performance liquid chromatography (HPLC) and matrix-assisted laser desorption ionization-time-of-flight/ time-of-flight (MALDI-TOF/TOF) mass spectrometry analysis with iTRAQ technique were performed. All data were analysed by MASCOT software with the SWISSPROT protein database. Furthermore, bioinformatics and statistical analysis were performed to classify their cellular components, biological processes, molecular functions and metabolic pathway by the PANTHER database.
Results:
In total, 137 differentially expressed proteins were identified during MCC growth and were assigned to one or more cellular components. According to the PANTHER analysis, a significant proportion of proteins are involved in the metabolic process, cellular process, biological regulation, developmental process and response to stimulus. The most extensive molecular function was 43% in catalytic activity. In addition, it was found that proteins in MCC chondrocytes change markedly on the growth stage of eruption of the teeth.
Conclusion:
This study provides an integrated perspective of molecular mechanisms regulating early normal postnatal growth and development of rat MCC at the protein level.
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