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Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy
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Gelsolin role in microapocrine secretion.

W Silva1, A F Ribeiro2, M C P Silva1

  • 1Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.

Insect Molecular Biology
|September 16, 2016
PubMed
Summary
This summary is machine-generated.

Gelsolin 1 facilitates microapocrine secretion in insects by severing actin filaments within microvilli, enabling digestive enzyme release. This research identifies gelsolin 1 as crucial for secretory vesicle transport.

Keywords:
RNA interferencegelsolinmicroapocrine secretionsecretory machinerysecretory vesicles

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Insect Physiology

Background:

  • Microapocrine secretion is a key process for releasing digestive enzymes in insects.
  • The molecular machinery underlying microapocrine secretion, particularly within microvilli, remains incompletely understood.

Purpose of the Study:

  • To investigate the role of specific proteins, including gelsolin 1, in the process of microapocrine secretion in insects.
  • To elucidate the function of gelsolin 1 in facilitating the transport of secretory vesicles along microvilli.

Main Methods:

  • Transcriptome analysis and literature searches to identify candidate proteins.
  • Cloning and sequencing of cDNAs for selected proteins (annexin, fimbrin, gelsolin 1, myosin 1, PDI 1, PDI 2).
  • Semi-quantitative reverse transcriptase PCR to determine tissue-specific expression.
  • Generation of antibodies against truncated gelsolin 1 for immunolocalization via electron microscopy.
  • RNA interference (RNAi) to suppress gelsolin 1 synthesis and observe effects on vesicle transport.

Main Results:

  • Gelsolin 1 and myosin 1 were found to be exclusively expressed in the insect midgut.
  • Immunoelectron microscopy confirmed the presence of gelsolin 1 within and around midgut cell microvilli.
  • Suppression of gelsolin 1 synthesis using RNAi blocked the advancement of secretory vesicles within microvilli.

Conclusions:

  • Gelsolin 1 plays a critical role in the machinery of microapocrine secretion.
  • Gelsolin 1 likely functions by severing actin filaments, thereby clearing a path for secretory vesicles within microvilli.