Acetyl pyridine-based palladium(II) compounds as an artificial metallonucleases

Area of Science:

• Coordination Chemistry
• Bioinorganic Chemistry
• Medicinal Chemistry

Background:

• Palladium complexes are investigated for their biological applications.
• 2-acetyl pyridine derivatives and benzene-12-dithiol are utilized as ligands.

Purpose of the Study:

• Synthesize and characterize novel Palladium(II) complexes.
• Investigate the interaction of these complexes with calf-thymus DNA (CT-DNA).
• Evaluate the antibacterial activity and DNA cleavage potential of the synthesized complexes.

Main Methods:

• Synthesis of Palladium(II) complexes: [Pd(L^n)Cl2] and [Pd(bdt)(L^n)].
• Characterization using TGA, elemental analysis, conductance, mass spectrometry, absorption, IR, 1H NMR, and EDX.
• DNA binding studies via absorption titration, viscosity measurements, fluorescence titration, and molecular modeling.
• Antibacterial activity screening against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Serratia marcescens, and Pseudomonas aeruginosa.
• DNA cleavage assay using gel electrophoresis.

Main Results:

• Palladium(II) complexes were successfully synthesized and characterized.
• Complexes exhibit an intercalative binding mode with CT-DNA, supported by spectroscopic and viscosity data.
• Fluorescence titration and molecular modeling further confirmed the intercalation.
• The complexes demonstrated antibacterial activity against tested pathogenic bacteria.
• All synthesized complexes showed pUC19 plasmid DNA cleavage ability.

Conclusions:

• The synthesized Palladium(II) complexes effectively bind to DNA via intercalation.
• These complexes possess significant antibacterial properties and DNA cleavage capabilities.
• The findings suggest potential applications of these complexes in medicinal chemistry and as antimicrobial agents.