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Two-photon polymerization setup enables experimental mapping and correction of spherical aberrations for improved
Optics Letters
|September 16, 2016
Summary
Two-photon polymerization can now create large, detailed structures by overcoming optical aberrations. This advancement enables the fabrication of macroscopic objects, including complex biomedical scaffolds, previously limited by microscope working distances.
Area of Science:
- Optics and Photonics
- Materials Science
- Biomedical Engineering
Background:
- Two-photon photopolymerization (TPP) allows sub-diffraction limit fabrication but is limited by optical aberrations and working distance.
- Fabricating macroscopic structures with TPP is challenging due to susceptibility to aberrations.
Purpose of the Study:
- To extend TPP for fabricating macroscopic, high-resolution structures.
- To develop a method for controlling optical aberrations during TPP.
- To enable the creation of large-scale, detailed biomedical scaffolds.
Main Methods:
- Utilized medium numerical-aperture microscope objectives for TPP.
- Implemented a movable substrate holder to maintain constant focusing conditions.
- Quantified spherical aberrations by mapping the optical point-spread function via polymerized voxels.
Main Results:
- Successfully fabricated macroscopic structures without limitations from objective working distance.
- Developed a method to monitor and minimize optical aberrations by analyzing voxel shapes.
- Demonstrated effective aberration control for fabricating large, detailed biomedical scaffolds with interconnected pores.
Conclusions:
- The developed TPP method overcomes previous limitations, enabling large-scale fabrication.
- Aberration control is crucial for high-fidelity TPP of macroscopic objects.
- This technique facilitates the production of advanced biomedical scaffolds for tissue engineering and regenerative medicine.
