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Quantitative phase imaging by single-shot Hilbert-Huang phase microscopy.

Maciej Trusiak, Vicente Mico, Javier Garcia

|September 16, 2016

Summary

We developed a new Hilbert-Huang transform algorithm for digital holographic microscopy. This method provides fast, accurate, single-shot quantitative phase imaging, outperforming existing techniques for biological samples.

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Area of Science:

Optics and Photonics
Biomedical Imaging
Digital Holography

Background:

Quantitative phase imaging (QPI) is crucial for label-free microscopy.
Digital holographic microscopy (DHM) offers high-resolution phase contrast imaging.
Existing QPI methods in DHM can be slow or require multiple measurements.

Purpose of the Study:

To introduce a novel single-shot algorithm for quantitative phase imaging using DHM.
To enhance robustness and accuracy in phase retrieval from fringe patterns.
To enable fast, single-acquisition phase imaging in both on-axis and off-axis DHM configurations.

Main Methods:

A single-shot Hilbert-Huang transform-based algorithm was developed.
Adaptive filtering and local fringe direction estimation were employed for fringe pattern analysis.
The algorithm was applied to digital holographic microscopy (DHM).

Main Results:

The proposed method achieved robust, fast, and accurate single-shot quantitative phase imaging.
Defective and closed fringe patterns were effectively handled.
Experimental results on microbeads and red blood cells showed favorable comparison with Fourier and temporal phase shifting methods.

Conclusions:

The Hilbert-Huang transform-based algorithm offers a significant advancement for single-shot QPI in DHM.
This technique provides a powerful tool for rapid, high-fidelity phase imaging of biological specimens.
The method demonstrates superior performance and adaptability for various DHM configurations.