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Updated: Mar 15, 2026

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
Published on: February 6, 2018
Generation of Human Antibodies Against a Specific Peptide: a Novel Strategy Based on Human Cells
Maria Toloudi1, Maria Papadimitriou1, Katerina Pantopikou1
1Research Genetic Cancer Centre Group, Florina, Greece.
Background/Aim:
We developed a novel platform to produce fully human antibodies that could be used as tools in cancer research. This study was based on maturation of B-cells into plasma cells and the subsequent secretion of fully human antibodies against a 9-mer peptide.
Materials And Methods:
A peptide was introduced to dendritic cells, followed by co-cultivation with cluster of differentiation 4 (CD4+) cells from healthy donors and CD19(+) cells from patients with cancer. In order to detect plasma cells, we used microscopy, flow cytometry, enzyme-linked immunosorbent assays (ELISAs), and western blotting.
Results:
Oval-shaped cells with eccentric nuclei were found which expressed high levels of CD27 (up to 73%) and CD138 (up to 31%). An antibody against the selected peptide was detected in the culture supernatants of all samples according to ELISA and western blot analyses.
Conclusion:
This platform successfully generated fully human antibodies against a specific epitope.
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