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Related Experiment Video

Updated: Mar 15, 2026

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Published on: November 29, 2014

Novel, high-quality surface plasmon resonance microscopy.

Rahber Thariani¹, Paul Yager¹

¹Department of Bioengineering, University of Washington, Seattle, WA 98195, United States.

Sensors and Actuators. B, Chemical

|September 16, 2016

Summary

A novel surface plasmon resonance microscope achieves high-quality, speckle-free imaging using an inexpensive laser and acoustic transducer to minimize artifacts. This design enhances imaging capabilities for various scientific applications.

Keywords:

Cost-effective imaging Laser pointer Speckle-free imaging Surface plasmon resonance microscopy

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Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

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Last Updated: Mar 15, 2026

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Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

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Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

Area of Science:

Optics and Photonics
Biophysics
Microscopy Technology

Background:

Surface plasmon resonance (SPR) microscopy is a powerful label-free imaging technique.
Image artifacts, such as speckle, can degrade the quality of SPR microscopy.
Inexpensive laser sources can introduce significant speckle, limiting their use in sensitive imaging.

Purpose of the Study:

To design and demonstrate a surface plasmon resonance microscope capable of high-quality, speckle-free imaging.
To investigate the use of an inexpensive acoustic transducer for artifact reduction.
To provide a cost-effective solution for advanced microscopy.

Main Methods:

Design of a novel surface plasmon resonance microscope.
Integration of a laser as the illumination source.
Incorporation of an inexpensive acoustic transducer to mitigate speckle and image artifacts.
Experimental demonstration of the system's operational capabilities.

Main Results:

Achieved high-quality, speckle-free imaging using the designed SPR microscope.
Successfully reduced image artifacts through the use of an acoustic transducer.
Demonstrated the effectiveness of an inexpensive laser pointer as an illumination source when combined with the acoustic transducer.

Conclusions:

The developed surface plasmon resonance microscope offers a viable method for high-quality, artifact-free imaging.
The use of an acoustic transducer is an effective strategy for speckle reduction in laser-based microscopy.
This cost-effective design expands the accessibility of advanced microscopy techniques.