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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
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High-Throughput Sequencing-Based Immune Repertoire Study during Infectious Disease.
Dongni Hou1, Cuicui Chen1, Eric John Seely2
1Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University , Shanghai , China.
Frontiers in Immunology
|September 16, 2016
Summary
Understanding the immune repertoire, the diverse collection of T and B cells, is key to fighting infectious diseases. Recent advances, especially high-throughput sequencing, offer new insights for diagnostics and therapies.
Area of Science:
- Immunology
- Infectious Diseases
- Genomics
Background:
- Adaptive immunity relies on diverse T and B cell receptors for pathogen recognition.
- The immune repertoire is a dynamic collection of lymphocytes reflecting immune selectivity.
- Studying the immune repertoire is crucial for understanding host responses to infections.
Purpose of the Study:
- To review recent advances in studying the immune repertoire in the context of infectious diseases.
- To highlight the impact of high-throughput sequencing (HTS) on immune repertoire analysis.
- To discuss the implications of these advances for diagnostics, immunotherapies, and vaccine development.
Main Methods:
- Review of traditional immunological techniques for immune repertoire analysis.
- Application of high-throughput sequencing (HTS) for large-scale lymphocyte receptor determination.
- Integration of HTS data to understand immunologic changes during pathogen challenge.
Main Results:
- HTS techniques provide unprecedented efficiency and scale in characterizing lymphocyte receptor diversity.
- Advances in methodology enhance the understanding of immune responses to pathogens.
- Immune repertoire studies reveal insights into host defense mechanisms.
Conclusions:
- High-throughput sequencing has revolutionized immune repertoire research in infectious diseases.
- Understanding immune repertoire dynamics is essential for developing novel medical countermeasures.
- This field holds significant promise for future diagnostic markers, immunotherapies, and vaccines.
