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Updated: Mar 15, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
The Role of Lysophospholipid Acyltransferases in the Golgi Complex
1Department of Biology, Villanova University, Mendel Hall Rm G63B, 800 Lancaster Avenue, Villanova, PA, 19085, USA. john.a.schmidt@villanova.edu.
Abstract:
Determining the abundance of phospholipids and neutral lipids in cellular membranes is paramount to understanding their biological functions. Many lipid-modifying enzymes have yet to be characterized due to limitations in substrate-product measurements and purification of membrane-bound enzymes. The method described here uses radiolabeled phospholipid substrates and cell-purified organelles to quantify phospholipid metabolism using thin-layer chromatography. This assay has the benefits of being specific and adaptable for numerous applications and systems.
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