Related Experiment Video
Updated: Mar 15, 2026
Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
Published on: March 14, 2019
MicroRNA-330-5p negatively regulates ITGA5 expression in human colorectal cancer
Hye-In Yoo1, Bong-Kyu Kim1, Sungjoo Kim Yoon1
1Department of Medical Life Sciences, The Catholic University of Korea, Seoul, Seochogu 137-701, Republic of Korea.
Abstract:
Colorectal cancer (CRC), one of the most prevalent malignant cancers, has high rates pf incidence and is the fourth leading cause of cancer-related deaths for both men and women worldwide. MicroRNAs (miRNAs) play critical roles in the development of various types of cancers. miRNA‑330-5p has been implicated in the progression of prostate, neuronal and pancreatic cancers by regulating proliferation, migration, invasion and epithelial-mesenchymal transition of cells. The purpose of the present study was to investigate the expression of miR-330-5p in CRC and identify its target gene(s) that may act in CRC tumorigenesis. We found that miR-330-5p expression was significantly lower in CRC tissues than that in adjacent non-tumorous tissues. Furthermore, we identified integrin α5 (ITGA5) as a new target of miR-330-5p and found that it inhibits ITGA5 expression by directly binding to the 3' untranslated region of ITGA5 mRNA. These results suggest that downregulation of miR-330-5p expression may affect CRC development via modulation of ITGA5 expression.
Insights
MicroRNA-330-5p is downregulated in colorectal cancer (CRC), suggesting a role in tumor development. This microRNA targets integrin α5 (ITGA5), potentially impacting CRC progression.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Colorectal cancer (CRC) is a leading cause of cancer-related mortality globally.
- MicroRNAs (miRNAs) are crucial regulators in cancer development.
- miRNA-330-5p is implicated in other cancers, affecting cell proliferation, migration, invasion, and epithelial-mesenchymal transition.
Purpose of the Study:
- To investigate the expression levels of miR-330-5p in colorectal cancer tissues.
- To identify novel target genes of miR-330-5p involved in colorectal cancer tumorigenesis.
Main Methods:
- Quantitative analysis of miR-330-5p expression in CRC tissues versus adjacent non-tumorous tissues.
- Identification of miR-330-5p targets through molecular binding assays.
- Validation of direct interaction between miR-330-5p and the 3' untranslated region (UTR) of target mRNA.
Main Results:
- miR-330-5p expression was significantly decreased in colorectal cancer tissues compared to normal adjacent tissues.
- Integrin α5 (ITGA5) was identified as a direct target of miR-330-5p.
- miR-330-5p suppresses ITGA5 expression by binding to its 3' UTR.
Conclusions:
- The downregulation of miR-330-5p may contribute to colorectal cancer development.
- Modulation of ITGA5 expression by miR-330-5p is a potential mechanism in CRC tumorigenesis.
Related Concept Videos
01:22MicroRNAs
01:22MicroRNAs
02:15Experimental RNAi