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Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
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ZNF606 interacts with Sox9 to regulate chondrocyte differentiation.

Zhenhai Zhou1, Honggui Yu1, Yunjia Wang1

  • 1Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China.

Biochemical and Biophysical Research Communications
|September 17, 2016
PubMed
Summary

The transcription factor ZNF606 interacts with Sox9, inhibiting chondrocyte differentiation. ZNF606 binding to Sox9 target gene enhancers decreases during differentiation, revealing its role as a novel Sox9 co-regulator.

Keywords:
Chondrocyte differentiationSox9ZNF606

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Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • The SRY-related high-mobility-group box 9 (Sox9) gene is crucial for chondrocyte differentiation and cartilage development.
  • Understanding the regulation of Sox9 activity is key to comprehending cartilage formation processes.

Purpose of the Study:

  • To identify novel interacting partners of Sox9.
  • To elucidate the role of ZNF606 in regulating Sox9 transcriptional activity and chondrocyte differentiation.

Main Methods:

  • Co-immunoprecipitation to identify interacting proteins.
  • Reporter gene assays to assess transcriptional activity.
  • Chromatin immunoprecipitation (ChIP) to determine DNA binding.
  • Gene knockdown and overexpression studies.

Main Results:

  • ZNF606 was identified as a novel interacting partner of Sox9.
  • Overexpression of ZNF606 inhibited Sox9 transcriptional activity, while ZNF606 knockdown enhanced it.
  • ZNF606 was shown to prevent Sox9 binding to the enhancers of the target gene col2a1.
  • The interaction between ZNF606 and Sox9 decreased during chondrocyte differentiation.
  • ZNF606 inhibited chondrocyte differentiation.

Conclusions:

  • ZNF606 acts as a novel co-regulator of Sox9.
  • ZNF606 negatively regulates Sox9-mediated chondrocyte differentiation by interfering with Sox9 binding to target gene enhancers.
  • The dynamic interaction between ZNF606 and Sox9 is important for the regulation of chondrogenesis.