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[Measuring chemiluminescence during blood preservation. 1. Spontaneous chemiluminescence]
Abstract:
A device for the measurement of spontaneous chemiluminescence (SCL) of biological fluids and cell suspensions according to Vladimirov (1966) description with using of contemporary equipment was constructed. Its dark impulse rate at room temperature of the photomultiplier was 1.5 s-1. The volume of measuring cell was 2 ml. An application of the device to investigate the blood during its conservation (21 days at 4 degrees C) showed an absence of the SCL of whole blood. The mean values of plasma SCL were in agreement with literature data. There were no regular changes of plasma SCL during the storage of blood indicating unsuitability of SCL for the detection of blood injury during its conservation.