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[15N] ammonium test for liver function diagnosis.

K Jung, H Faust, R Matkowitz

    Zeitschrift Fur Medizinische Laboratoriumsdiagnostik
    |January 1, 1989
    PubMed
    Summary
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    Assessing liver function is possible using 15N labelled ammonium chloride. Urine analysis of [15N]urea and [15N]ammonia ratios after tracer intake effectively differentiates liver disease patients from healthy individuals.

    Area of Science:

    • Biochemistry
    • Medical Diagnostics
    • Isotope Tracing

    Background:

    • Liver function is crucial for metabolic processes.
    • Accurate assessment of liver health is vital for timely diagnosis and treatment.
    • Current diagnostic methods may have limitations.

    Purpose of the Study:

    • To evaluate the efficacy of 15N labelled ammonium chloride as a tracer for assessing liver function.
    • To determine if urinary [15N]urea and [15N]ammonia ratios can distinguish liver disease patients from healthy subjects.

    Main Methods:

    • Oral administration of 15N labelled ammonium chloride (tracer) to subjects.
    • Isotope analysis of [15N]urea and [15N]ammonia in urine samples collected 3 hours post-administration.
    • Calculation of the ratio of excess abundances of [15N]ammonia to [15N]urea.

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    Main Results:

    • The ratio of [15N]ammonia to [15N]urea in urine showed significant differences between patients with liver diseases and healthy subjects.
    • This isotopic ratio serves as a reliable indicator of impaired liver function.

    Conclusions:

    • Oral 15N labelled ammonium chloride administration followed by urinary isotope analysis is a viable method for liver function assessment.
    • The [15N]ammonia/[15N]urea ratio in urine is a sensitive biomarker for detecting liver diseases.