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Updated: Mar 15, 2026

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS
Published on: October 17, 2010
Highly Sensitive Coherent Anti-Stokes Raman Scattering Imaging of Protein Crystals
Grigory M Arzumanyan1,2, Nelya V Doroshkevich1, Kahramon Z Mamatkulov1
1Joint Institute for Nuclear Research , 141980 Dubna, Russia.
Abstract:
Serial crystallography at last generation X-ray synchrotron sources and free electron lasers enabled data collection with micrometer and even submicrometer size crystals, which have resulted in amazing progress in structural biology. However, imaging of small crystals, which although is highly demanded, remains a challenge, especially in the case of membrane protein crystals. Here we describe a new extremely sensitive method of the imaging of protein crystals that is based on coherent anti-Stokes Raman scattering.
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