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Updated: Mar 15, 2026
Surgical Models of Gastroesophageal Reflux with Mice
Published on: August 25, 2015
Decreased n-6/n-3 polyunsaturated fatty acid ratio reduces chronic reflux esophagitis in rats
Jing-Jing Wei1, Du-Peng Tang1, Jing-Jing Xie1
1Department of Gastroenterology, the First Affiliated Hospital of Fujian Medical University, China.
Aim:
To investigate the effect of dietary ratio of n-6/n-3 PUFAs on chronic reflux esophagitis (RE) and lipid peroxidation.
Method:
Rat RE model were established and then fed on a diet contained different n-6/n-3 PUFA ratios (1:1.5, 5:1, 10:1) or received pure n-6 PUFA diet for 14 days. Esophageal pathological changes were evaluated using macroscopic examination and hematoxyline-eosin staining. IL-1β, IL-8, and TNFα mRNA and protein levels of were determined using RT-PCR and Western blotting, respectively. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were determined using ELISA.
Results:
The severity of esophagitis was lowest in the PUFA(1:1.5) group (P<0.05). IL-1β, IL-8, and TNFα mRNA and protein and MDA levels were significantly increased in model groups with the increasing n-6/n-3 PUFA ratios. SOD levels were significantly decreased in all RE PUFA groups (P<0.05).
Conclusion:
Esophageal injury and lipid peroxidation appeared to be ameliorated by increased n-3 PUFAs intake.
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