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Oleaginous yeasts from Ethiopia.

Tamene Milkessa Jiru1, Dawit Abate2, Nicholas Kiggundu3

  • 1Microbial, Cellular and Molecular Biology Department, College of Natural Sciences, Addis Ababa University, P.O. Box: 1176, Addis Ababa, Ethiopia. tamene.milkessa@aau.edu.et.

AMB Express
|September 18, 2016
PubMed
Summary

Researchers isolated 18 oleaginous yeast strains from Ethiopian environments for biodiesel production. Cutaneotrichosporon curvatus PY39 showed the highest lipid yield, making it a promising candidate for future biofuel applications.

Keywords:
BiodieselOleaginous yeastSingle cell oilYeast identification

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Area of Science:

  • Microbiology
  • Biotechnology
  • Renewable Energy

Background:

  • Oleaginous microorganisms are key for sustainable oil production.
  • Ethiopia's diverse environments offer potential for discovering novel oleaginous yeasts.
  • Lipid-producing yeasts are a sustainable feedstock for biodiesel.

Purpose of the Study:

  • To isolate and identify oleaginous yeasts from various Ethiopian sources.
  • To characterize the lipid production capabilities of isolated yeast strains.
  • To evaluate the potential of these yeasts as a sustainable source for biodiesel production.

Main Methods:

  • Sample collection from soil, plants, waste oils, and dairy products in Ethiopia.
  • Isolation and screening of yeast colonies using Sudan III staining.
  • Identification of yeast strains via morphological, physiological, and molecular methods (rRNA gene sequencing).
  • Cultivation under nitrogen-limited conditions to assess biomass and lipid production.

Main Results:

  • 18 oleaginous yeast strains were isolated and identified, including Cutaneotrichosporon curvatus, Rhodotorula kratochvilovae, Rhodotorula dairenensis, and Rhodotorula mucilaginosa.
  • Cutaneotrichosporon curvatus PY39 achieved the highest lipid yield (6.87 g/L) and lipid content (46.51%).
  • Rhodotorula mucilaginosa strains exhibited varying biomass and lipid production levels.

Conclusions:

  • Ethiopian environments harbor a significant number of oleaginous yeasts with potential for biodiesel production.
  • Cutaneotrichosporon curvatus PY39 and two Rhodotorula mucilaginosa strains (SY89, SY18) are recommended for further optimization.
  • This study highlights the potential of indigenous yeast strains for sustainable biofuel development.