Novel yeast isolated from broilers' feedstuff, gut and faeces as aflatoxin B₁ adsorbents

Area of Science:

• Animal Science
• Microbiology
• Food Safety

Background:

• Aflatoxin B1 (AFB1) is a toxic secondary metabolite produced by Aspergillus species, posing a significant threat to poultry health and productivity.
• Chronic aflatoxicosis in avian populations can lead to reduced growth, impaired immunity, and economic losses in the poultry industry.

Purpose of the Study:

• To isolate and identify native yeast strains from broiler environments (feedstuff, feces, gut).
• To evaluate the aflatoxin B1 (AFB1) binding capacity of these isolated yeast strains.
• To assess the potential of native yeasts as natural adsorbents for AFB1 in poultry feed.

Main Methods:

• Isolation and identification of yeast strains from various broiler environmental sources.
• In vitro assessment of AFB1 binding percentages across different yeast strains and AFB1 concentrations.
• Adsorption isotherm studies (Langmuir type) and stability analysis of AFB1-yeast complexes.

Main Results:

• Nine yeast strains were isolated, including Pichia kudriavzevii, Clavispora lusitaniae, Candida tropicalis, and Cyberlindnera fabianii.
• All tested yeast strains demonstrated AFB1 binding capacity, with optimal conditions at 10^7 cells/mL and 100 ng/mL AFB1.
• Adsorption followed Langmuir-type isotherms, and the AFB1-yeast complex formation was stable and irreversible.

Conclusions:

• Native yeast strains from broiler environments are effective AFB1 adsorbents.
• These yeasts offer a promising, naturally occurring strategy for mitigating aflatoxicosis in poultry production.
• The study contributes to developing sustainable methods for controlling mycotoxin contamination in avian diets.