Related Experiment Video
Updated: Mar 15, 2026

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
Published on: April 28, 2017
Fast Fluorescence Microscopy with Light Sheets
Stephan Daetwyler1, Jan Huisken2
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
Abstract:
In light sheet microscopy, optical sectioning by selective fluorescence excitation with a sheet of light is combined with fast full-frame acquisition. This illumination scheme provides minimal photobleaching and phototoxicity. Complemented with remote focusing and multi-view acquisition, light sheet microscopy is the method of choice for acquisition of very fast biological processes, large samples, and high-throughput applications in areas such as neuroscience, plant biology, and developmental biology. This review explains why light sheet microscopes are much faster and gentler than other established fluorescence microscopy techniques. New volumetric imaging schemes and highlights of selected biological applications are also discussed.
Related Concept Videos
Super-resolution Fluorescence Microscopy
Confocal Fluorescence Microscopy
Total Internal Reflection Fluorescence Microscopy
Two-Dimensional Microscopy in Microbiology

