Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

ATP Energy Storage and Release01:31

ATP Energy Storage and Release

15.1K
ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
One example of energy coupling using ATP involves a...
15.1K
ATP Energy Storage and Release01:31

ATP Energy Storage and Release

3.1K
3.1K
Hydrolysis of ATP01:08

Hydrolysis of ATP

82.9K
The bonds of adenosine triphosphate (ATP) can be broken through the addition of water, releasing one or two phosphate groups in an exergonic process called hydrolysis. This reaction liberates the energy in the bonds for use in the cell—for instance, to synthesize proteins from amino acids.
If one phosphate group is removed, a molecule of ADP—adenosine diphosphate—remains, along with inorganic phosphate. ADP can be further hydrolyzed to AMP—adenosine...
82.9K
Hydrolysis of ATP01:08

Hydrolysis of ATP

8.0K
8.0K
ATP and Energy Production01:23

ATP and Energy Production

2.5K
Adenosine triphosphate (ATP) is a critical molecule that functions as the main energy carrier in cells. Structurally, ATP consists of an adenosine molecule—comprising adenine and ribose—bonded to three phosphate groups. The high-energy bonds between these phosphate groups store significant amounts of potential energy. This energy is released during hydrolysis, wherein ATP is converted to adenosine diphosphate (ADP) or adenosine monophosphate (AMP), driving a variety of essential...
2.5K
Positron Emission Tomography01:29

Positron Emission Tomography

7.9K
Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
7.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial Voltage-Dependent Anion Channel: From a Passive Pore to a Cellular Hub Through Protein Complexation.

International journal of molecular sciences·2026
Same author

Identifying Novel Estrogenic Mitochondrial Targets in Hypothalamic Proopiomelanocortin Neurons by Chemoproteomics.

bioRxiv : the preprint server for biology·2026
Same author

mSumireF a Monomeric Violet Fluorescent Protein.

ACS bio & med chem Au·2026
Same author

Three mammalian VDAC isoforms distinctly regulate mitochondrial function and proteome to maintain cell metabolism.

The Journal of biological chemistry·2025
Same author

R103 and R115 Affinity Mutants of ATeam ATP Biosensors.

Sensors (Basel, Switzerland)·2025
Same author

An Improved Ratiometric FRET Biosensor with Higher Affinity for Extracellular ATP.

Sensors (Basel, Switzerland)·2025

Related Experiment Video

Updated: Mar 15, 2026

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK
11:20

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK

Published on: December 19, 2019

10.6K

Imaging Adenosine Triphosphate (ATP).

Megha Rajendran1, Eric Dane2, Jason Conley1

  • 1Department of Chemistry, Purdue University, 560 Oval Drive, Box 68, West Lafayette, Indiana 47907; and.

The Biological Bulletin
|September 18, 2016
PubMed
Summary

Adenosine triphosphate (ATP) dynamics are crucial for cellular functions. New imaging technologies are needed to visualize ATP in living cells across different scales for deeper mechanistic insights.

More Related Videos

Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice
08:31

Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice

Published on: June 30, 2021

3.2K
Assaying Protein Kinase Activity with Radiolabeled ATP
08:05

Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

19.5K

Related Experiment Videos

Last Updated: Mar 15, 2026

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK
11:20

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK

Published on: December 19, 2019

10.6K
Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice
08:31

Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice

Published on: June 30, 2021

3.2K
Assaying Protein Kinase Activity with Radiolabeled ATP
08:05

Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

19.5K

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Physiology

Background:

  • Adenosine triphosphate (ATP) is a universal mediator of cellular metabolism and signaling.
  • ATP dynamics are fundamentally linked to cellular physiology, both internally and externally.
  • Understanding ATP dynamics offers mechanistic insights into vital processes like neurotransmission and immune cell chemotaxis.

Purpose of the Study:

  • To review current technologies for visualizing ATP in living cells.
  • To identify limitations and future needs in ATP visualization methodologies.
  • To enable interrogation of temporal and spatial ATP dynamics from subcellular to organismal levels.

Main Methods:

  • Development of ATP-specific molecular probes.
  • Integration of molecular probes with advanced imaging techniques, particularly optical microscopy.
  • Qualitative and quantitative detection of ATP in live specimens.

Main Results:

  • A survey of existing technologies for visualizing ATP in living cells was conducted.
  • The review highlights the combination of ATP probes with optical microscopy for detection.
  • Current methods enable qualitative and quantitative assessment of ATP distribution.

Conclusions:

  • Existing molecular probes and imaging techniques allow for ATP visualization in live cells.
  • There is a need for novel tools and approaches to enhance the capabilities of ATP dynamics interrogation.
  • Advancements are required to fully capture temporal and spatial ATP dynamics across biological scales.