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Updated: Mar 15, 2026

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes
Published on: May 4, 2018
Charge translocation by mitochondrial NADH:ubiquinone oxidoreductase (complex I) from Yarrowia lipolytica measured on
1Molecular Bioenergetics Group, Medical School, Johann Wolfgang Goethe-University, 60590, Frankfurt am Main, Germany; Goethe University Frankfurt, Institute of Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Protein Reaction Control Group, Max-von-Laue-Str. 15, 60438, Frankfurt am Main, Germany.
Abstract:
The charge translocation by purified reconstituted mitochondrial complex I from the obligate aerobic yeast Yarrowia lipolytica was investigated after adsorption of proteoliposomes to solid-supported membranes. In presence of n-decylubiquinone (DBQ), pulses of NADH provided by rapid solution exchange induced charge transfer reflecting steady-state pumping activity of the reconstituted enzyme. The signal amplitude increased with time, indicating 'deactive→active' transition of the Yarrowia complex I. Furthermore, an increase of the membrane-conductivity after addition of 5-(N-ethyl-N-isopropyl)amiloride (EIPA) was detected which questiones the use of EIPA as an inhibitor of the Na+/H+-antiporter-like subunits of complex I. This investigation shows that electrical measurements on solid-supported membranes are a suitable method to analyze transport events and 'active/deactive' transition of mitochondrial complex I.
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