Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

6.5K
Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...
6.5K
Factors Affecting Erythropoiesis01:24

Factors Affecting Erythropoiesis

6.6K
The cardiovascular system regulates the number of erythrocytes in the bloodstream to ensure optimal oxygen transport. It also prevents over-proliferation of these cells, which helps to maintain blood viscosity and flow rate.
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...
6.6K
Erythropoiesis01:14

Erythropoiesis

6.5K
Red blood cells  (RBCs) transport oxygen to all body tissues. These cells survive only for 120 days and then need to be replenished. Erythropoiesis is the process of RBC production. In healthy individuals, erythropoiesis ensures all tissues are amply supplied with oxygen. In addition, blood loss due to injury leads to a drop in the physiological oxygen level that will cause erythropoiesis. Any defect in erythropoiesis leads to several physiological disorders, including thalassemia, anemia,...
6.5K
Erythropoiesis01:14

Erythropoiesis

3.9K
3.9K
Structure and Function of Erythrocytes01:29

Structure and Function of Erythrocytes

7.6K
There are between 4.2 and 6 million erythrocytes, also known as red blood cells, in every microliter of blood. These cells are small, flattened biconcave discs with centers that are depressed.
The erythrocyte plasma membrane is associated with proteins such as spectrin, which forms a flexible cytoplasmic meshwork. This meshwork allows erythrocytes to twist, turn, become cup-shaped, and regain their biconcave shape as they pass through narrow capillaries. Additionally, erythrocytes can form...
7.6K
Paracrine Signaling01:21

Paracrine Signaling

60.2K
Paracrine signaling allows cells to communicate with their immediate neighbors via secretion of signaling molecules. Such a signal can only trigger a response in nearby target cells because the signal molecules degrade quickly or are inactivated if not taken up. Prominent examples of paracrine signaling include nitric oxide signaling in blood vessels, synaptic signaling of neurons, the blood clotting system, tissue repair/wound healing, and local allergic skin reactions. Nitric oxide as a...
60.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunomodulatory endothelial cells contribute to T cell recruitment and activation via antigen presentation on MHC II.

Cardiovascular research·2026
Same author

A microprotein encoded by FERMT3 modulates endothelial cell protein catabolism and induces cell cycle arrest and senescence.

Cell communication and signaling : CCS·2026
Same author

IRG1/itaconate rewires macrophage and lung tumor metabolism through G6PD inhibition.

Cell metabolism·2026
Same author

Pathophysiology, prevention, and management of coronary microvascular obstruction.

European heart journal·2026
Same author

Soluble epoxide hydrolase drives neurovascular dysfunction in a model of amyloidosis.

Brain : a journal of neurology·2026
Same author

Identification of unannotated microproteins involved in endothelial cell homeostasis, dysfunction, and vascular disease.

Cardiovascular research·2026

Related Experiment Video

Updated: Mar 15, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

4.9K

Can erythrocytes release biologically active NO?

Peter M Benz1,2, Ingrid Fleming3,4

  • 1Institute for Vascular Signalling, Centre for Molecular Medicine, Johann Wolfgang Goethe University, Frankfurt, Germany. benz@vrc.uni-frankfurt.de.

Cell Communication and Signaling : CCS
|September 19, 2016
PubMed
Summary
This summary is machine-generated.

Erythrocytes (red blood cells) do not produce nitric oxide (NO) to inhibit platelet activation, according to a new study. Researchers found no evidence of NO signaling from red blood cells affecting platelet function.

Keywords:
ErythrocytesHypoxic vasodilationNONitric oxidePKGPlatelet inhibitionRed blood cellsSoluble guanylyl cyclaseVASPeNOS

More Related Videos

Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins
10:07

Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins

Published on: March 17, 2023

2.5K
Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

18.6K

Related Experiment Videos

Last Updated: Mar 15, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

4.9K
Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins
10:07

Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins

Published on: March 17, 2023

2.5K
Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

18.6K

Area of Science:

  • Cardiovascular Physiology
  • Cellular Signaling

Background:

  • Endothelial cells and endothelial nitric oxide synthase (eNOS) are primary sources of nitric oxide (NO) in the cardiovascular system.
  • Erythrocytes have been controversially proposed to regulate cell functions via NO release, including platelet inhibition.

Purpose of the Study:

  • To investigate whether erythrocytes release biologically active nitric oxide (NO) capable of inhibiting platelet activation.

Main Methods:

  • Assayed NO production by erythrocytes by monitoring activation of platelet soluble guanylyl cyclase (sGC) and protein kinase G (PKG).
  • Systematically tested various combinations of erythrocyte and platelet suspensions.

Main Results:

  • No evidence of platelet sGC/PKG activation by erythrocytes was found.
  • Erythrocytes did not demonstrate the release of NO to affect platelet function.

Conclusions:

  • Erythrocytes do not appear to release biologically active NO that inhibits platelet activation.
  • The proposed role of NO derived from red blood cells in regulating platelet function is not supported by this study.