Phenotypic plasticity to altered apical bud temperature in Cucumis sativus: more leaves-smaller leaves and vice versa

Many studies investigated temperature effects on leaf initiation and expansion by relating these processes to air temperature or the temperature of a specific organ (e.g. leaf temperature). In reality plant temperature is hardly ever equal to air temperature or spatially uniform. Apical bud temperature (T_bud ), for example, may greatly differ from the temperature of the rest of the plant (T_plant ) dependent on the environment. Recent research in Cucumis sativus showed that T_bud influences leaf initiation independent of T_plant . These findings trigger the question if such spatial temperature differences also influence leaf expansion and plant phenotype. In a 28 day study, we maintained temperature differences between T_bud and T_plant ranging from -7 to +8 °C using a custom-made bud temperature control system. Leaf expansion did not only depend on leaf temperature but also on the difference between bud and leaf temperature. Differences between T_bud and T_plant considerably influenced vertical leaf area distribution over the shoot: increasing T_bud beyond T_plant resulted in more and smaller leaves, while decreasing T_bud below T_plant resulted in less and larger leaves. The trade-off between leaf number and leaf area resulted in phenotypic alterations that cannot be predicted, for example, by crop models, when assuming plant temperature uniformity.