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Evaluation of Glutathione Peroxidase 4 role in Preeclampsia
Xinguo Peng1, Yan Lin2, Jinling Li1
1Clinical Laboratory, Binzhou Medical University Hospital, Binzhou, China.
Insights
Genetic variations in Glutathione Peroxidase 4 (GPx4), specifically the rs713041 polymorphism, are associated with an increased risk of preeclampsia (PE) in Chinese Han women. This finding highlights GPx4
Area of Science:
- Genetics
- Obstetrics
- Biochemistry
Background:
- Preeclampsia (PE) is a serious pregnancy complication.
- Oxidative stress is implicated in PE pathogenesis.
- Glutathione Peroxidase 4 (GPx4) is a key antioxidant enzyme.
Purpose of the Study:
- To investigate the association between GPx4 gene polymorphisms and PE susceptibility in Chinese Han women.
- To explore the role of GPx4 in PE development.
Main Methods:
- Genotyping of GPx4 polymorphisms (rs713041 and rs4807542) using TaqMan allelic discrimination real-time PCR.
- Case-control study involving 1008 PE patients and 1386 controls.
Main Results:
- Significant differences in genotypic and allelic frequencies of rs713041 were observed between PE patients and controls.
- The C allele of rs713041 was associated with a higher risk of PE.
- rs713041 genotype was linked to mild, severe, and early-onset PE.
Conclusions:
- The rs713041 polymorphism in the GPx4 gene may play a significant role in the pathogenesis of preeclampsia.
- GPx4 genetic variations could be potential biomarkers for PE risk assessment.
Abstract:
Preeclampsia (PE) is a pregnancy-specific syndrome that may be lifethreatening to pregnancies and fetus. Glutathione Peroxidase 4 (GPx4) is a powerful antioxidant enzyme that can provide protection from oxidative stress damage which plays a pivotal role in the pathology of PE. Therefore, this study aims to investigate the association between Gpx4 polymorphisms and the susceptibility to PE in Chinese Han women. TaqMan allelic discrimination real-time PCR was used to perform the genotyping of rs713041 and rs4807542 in 1008 PE patients and 1386 normotensive pregnancies. Obviously statistical difference of genotypic and allelic frequencies were found of rs713041 in GPx4 between PE patients and controls and the C allele has the higher risk for pathogenesis of PE (χ(2) = 12.292, P = 0.002 by genotype; χ(2) = 11.035, P = 0.001, OR = 1.216, 95% CI 1.084-1.365 by allele). Additionally, when subdividing these samples into CC + CT and TT groups, we found a significant difference between the two groups (χ(2) = 11.241, P = 0.001, OR = 1.417, 95% CI 1.155-1.738). Furthermore, the genotype of rs713041 was found to be associated with the mild, severe and early-onset PE. Our results suggest that rs713041 in GPx4 may play a key role in the pathogenesis of PE.
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