Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

2.8K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
2.8K
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

4.7K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
4.7K
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

2.4K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The NMR Exchange Format (NEF): Specification and Applications.

bioRxiv : the preprint server for biology·2026
Same author

NMRhub: An NMR Data Ecosystem Spanning the Complete Data Lifecycle.

Journal of molecular biology·2026
Same author

Securing the Future of NMR Metabolomics Reproducibility: A Call for Standardized Reporting.

Analytical chemistry·2025
Same author

Best Practices in NMR Metabolomics: Current State.

Trends in analytical chemistry : TRAC·2025
Same author

Author Correction: Emergence of structures in neuronal network activities.

Scientific reports·2025
Same author

Emergence of structures in neuronal network activities.

Scientific reports·2025

Related Experiment Video

Updated: Mar 14, 2026

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

1.4K

Increasing rigor in NMR-based metabolomics through validated and open source tools.

Hamid R Eghbalnia1, Pedro R Romero1, William M Westler1

  • 1Biochemistry Department, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706, USA.

Current Opinion in Biotechnology
|September 20, 2016
PubMed
Summary

Metabolomics, the study of small molecules, is crucial for understanding organism function and disease. This review highlights how databases and software tools enhance the reliability and reproducibility of metabolomics research.

More Related Videos

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.9K
An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome
05:35

An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome

Published on: September 20, 2022

4.3K

Related Experiment Videos

Last Updated: Mar 14, 2026

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

1.4K
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.9K
An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome
05:35

An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome

Published on: September 20, 2022

4.3K

Area of Science:

  • Metabolomics
  • Systems Biology
  • Biomarker Discovery

Background:

  • The metabolome, comprising small molecules, offers insights into organismal phenotype and functional states.
  • Metabolomics identifies and quantifies metabolites in biological samples, aiding various research areas.
  • Applications span systems biology, disease diagnostics, and biomarker discovery.

Purpose of the Study:

  • To review the critical role of databases and software tools in advancing metabolomics.
  • To emphasize the impact of these resources on research rigor, robustness, reproducibility, and validation.

Main Methods:

  • Utilizes information from nuclear magnetic resonance (NMR) and mass spectrometry (MS).
  • Integrates data from published literature.
  • Employs statistical approaches for data processing and analysis.

Main Results:

  • Databases and software tools are essential for high-throughput metabolomics data analysis.
  • These resources improve the accuracy and consistency of metabolite identification and quantification.
  • Advancements in computational tools drive wider applications of metabolomics.

Conclusions:

  • Databases and software are indispensable for robust and reproducible metabolomics studies.
  • Continued development of these tools will further enhance the field's capabilities.
  • This review underscores their importance for reliable biomarker discovery and disease diagnostics.