Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

9.8K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
9.8K
Mass Spectrometers01:16

Mass Spectrometers

10.1K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
10.1K
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

2.0K
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
2.0K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.8K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.8K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.0K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.0K
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

3.8K
An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid Classification of Coffee Varieties Using Single-Bean Hot Gas Extraction Ion-Mobility Spectrometry with Machine Learning.

ACS measurement science au·2026
Same author

Electrophoresis-By-Accident in Electrospray Ion Source.

Analytical chemistry·2026
Same author

Epidermal Patch Technologies for Integrated Healthcare and Infection Management.

Advanced healthcare materials·2026
Same author

Dynamic Additive Scanning for Precise Control in Electrospray Ionization Mass Spectrometry.

ACS measurement science au·2026
Same author

Negative Gaseous Ions in Positive-Voltage Electrospray Ionization Mass Spectrometry.

Analytical chemistry·2026
Same author

Dual Ionization Ion-Mobility Mass Spectrometry Hyphenated with Catalytic Oxygenation-Mediated Extraction.

ACS measurement science au·2026
Same journal

Inverse FIP effect plasma in the solar atmosphere: a synthesis of current understanding and new insights from AR 11967.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026
Same journal

Signs of sulfur fractionation under high magnetic field strength.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026
Same journal

First ionization potential fractionation of sulfur observed with spectral imaging of the coronal environment.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026
Same journal

Chromospheric dynamics and turbulence regulate the solar FIP effect.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026
Same journal

Exploring the link between wave activity in the photospheric velocity driver and the FIP bias in the solar corona.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026
Same journal

Radiative hydrodynamic simulations of first ionization potential fractionation in solar flares.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026
See all related articles

Related Experiment Video

Updated: Mar 14, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.9K

Quantitative mass spectrometry: an overview.

Pawel L Urban1

  • 1Department of Applied Chemistry, National Chiao Tung University, 1001 University Road, Hsinchu City 300, Taiwan plurban@nctu.edu.tw.

Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
|September 21, 2016
PubMed
Summary
This summary is machine-generated.

Mass spectrometry (MS) is a vital chemical analysis tool for molecular identification. This theme issue explores advancements making MS a fully quantitative measurement platform for research and industry.

Keywords:
bioanalysiscalibrationchemical analysismass spectrometryquantification

More Related Videos

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.7K
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

6.3K

Related Experiment Videos

Last Updated: Mar 14, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.9K
Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.7K
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

6.3K

Area of Science:

  • Analytical Chemistry
  • Metrology
  • Molecular Spectroscopy

Background:

  • Mass spectrometry (MS) is a cornerstone technique in modern chemistry, physics, and biochemistry.
  • Widely employed in research and industry (drug discovery, quality control, food safety) for molecular identification.
  • Current MS applications are primarily qualitative, relying on mass-to-charge ratios and fragmentation patterns.

Purpose of the Study:

  • To highlight technological and methodological advancements enabling quantitative mass spectrometry.
  • To foster fundamental and applied research utilizing quantitative MS measurements.
  • To provide insights from experts in the field of quantitative MS.

Main Methods:

  • Focus on improvements in mass spectrometry instrumentation and methodologies.
  • Discussion of techniques for precise molecular quantification using MS.
  • Expert contributions detailing practical applications and theoretical underpinnings.

Main Results:

  • Demonstration of MS as a robust platform for quantitative molecular measurements.
  • Validation of MS capabilities beyond qualitative analysis.
  • Showcasing the indispensable nature of MS in various scientific and industrial sectors.

Conclusions:

  • Mass spectrometry is evolving into a fully quantitative metrological tool.
  • Quantitative MS advancements are crucial for scientific discovery and industrial applications.
  • The theme issue serves as a resource for researchers and practitioners in quantitative MS.