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Updated: Mar 14, 2026

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight MALDI-TOF Mass Spectrometry
Published on: June 10, 2018
Critical factors determining the quantification capability of matrix-assisted laser desorption/ionization-
Chia-Chen Wang1, Yin-Hung Lai2, Yu-Meng Ou3
1Department of Biochemistry, National Yang-Ming University, No. 155, Section 2, Linong Street, Taipei 112, Taiwan, Republic of China Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Taipei 115, Taiwan, Republic of China.
Abstract:
Quantitative analysis with mass spectrometry (MS) is important but challenging. Matrix-assisted laser desorption/ionization (MALDI) coupled with time-of-flight (TOF) MS offers superior sensitivity, resolution and speed, but such techniques have numerous disadvantages that hinder quantitative analyses. This review summarizes essential obstacles to analyte quantification with MALDI-TOF MS, including the complex ionization mechanism of MALDI, sensitive characteristics of the applied electric fields and the mass-dependent detection efficiency of ion detectors. General quantitative ionization and desorption interpretations of ion production are described. Important instrument parameters and available methods of MALDI-TOF MS used for quantitative analysis are also reviewed.This article is part of the themed issue 'Quantitative mass spectrometry'.
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