Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.0K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.0K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.8K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.8K
Quantitative Analysis01:12

Quantitative Analysis

1.7K
Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...
1.7K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

7.4K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.4K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.8K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.8K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

9.8K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
9.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The discovery of α-glucosidase inhibitors from Folium Mori using virtual screening and ligand fishing strategies.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same author

Comparing the Degradation Pathways of Hydrochlorothiazide and Sulfamethoxazole Using Ozone, Anodic Oxidation, and Electro-Fenton Processes.

ACS ES&T water·2026
Same author

Exploring urinary allantoin and adenosine in pediatric autosomal dominant polycystic kidney disease: no clear association with disease severity.

Pediatric nephrology (Berlin, Germany)·2026
Same author

Green Chemistry 2026.

Electrophoresis·2026
Same author

A hydrophilic interaction liquid chromatography-tandem mass spectrometry method for the quantification of intracellular cystine and its application in cystinosis research.

Analytical and bioanalytical chemistry·2026
Same author

Microporous organic network immobilized α-glucosidase for inhibitors screening from Chrysanthemum morifolium Ramat extract and network pharmacology study.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026

Related Experiment Video

Updated: Mar 14, 2026

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

10.7K

Quantitative mass spectrometry methods for pharmaceutical analysis.

Glenn Loos1, Ann Van Schepdael1, Deirdre Cabooter2

  • 1KU Leuven, Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, Herestraat 49, 3000 Leuven, Belgium.

Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
|September 21, 2016
PubMed
Summary
This summary is machine-generated.

Quantitative mass spectrometry enhances pharmaceutical analysis using advanced techniques like liquid chromatography and electrospray ionization. This study explores methods to improve sensitivity and minimize interferences for reliable data in bioanalysis and environmental monitoring.

Keywords:
hyphenated techniquesinternal standardsmass spectrometrymatrix effectspharmaceutical analysissample preparation

More Related Videos

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

10.4K
Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
10:44

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Published on: March 28, 2017

10.3K

Related Experiment Videos

Last Updated: Mar 14, 2026

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

10.7K
High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

10.4K
Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
10:44

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Published on: March 28, 2017

10.3K

Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Pharmacology

Background:

  • Mass spectrometry is crucial for quantitative pharmaceutical analysis.
  • Electrospray ionization, triple quadrupole mass spectrometry, solid-phase extraction, and liquid chromatography are common techniques.
  • Isotopically labeled standards are used to manage ion suppression.

Purpose of the Study:

  • To present approaches for enhancing ionization efficiency and ion guidance.
  • To describe methods for minimizing, assessing, and correcting matrix interferences.
  • To discuss separation techniques and sample preparation for improved sensitivity in pharmaceutical analysis.

Main Methods:

  • Modified source geometries and improved ion guidance for ionization enhancement.
  • Strategies to minimize, assess, and correct matrix interferences from co-eluting substances.
  • Exploration of liquid chromatography trends and sample preparation methods.

Main Results:

  • Enhanced ionization efficiencies through modified source geometries and ion guidance.
  • Effective methods for managing matrix effects and interferences.
  • Discussion of techniques applicable to environmental and bioanalytical pharmaceutical analysis.

Conclusions:

  • Optimizing instrumentation and sample preparation is key for sensitive and reliable quantitative mass spectrometry.
  • Miniaturized systems offer potential for in-field applications in pharmaceutical analysis.
  • The study provides insights into improving quantitative data quality for pharmaceuticals.