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Interferometric Volume Measurement in Microplates.

Hans-Christian Luedemann1

  • 11 Meniscense LLC, Bolton, MA, USA.

SLAS Technology
|September 21, 2016
PubMed
Summary
This summary is machine-generated.

Low-coherence interferometry offers precise, noncontact measurement of liquid volumes and fill heights in microplates. This new method achieves high resolution and accuracy, comparable to existing systems but with enhanced capabilities.

Keywords:
high-throughput screeninginstrumentation qualificationrobotics and instrumentationworkflow

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Area of Science:

  • Metrology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Accurate liquid volume and fill height measurements are crucial in microplate-based assays.
  • Existing noncontact methods like ultrasonic ranging have limitations in resolution.
  • Ratiometric photometry is a high-resolution commercial standard but may have specific limitations.

Purpose of the Study:

  • To introduce and evaluate a novel low-coherence interferometry system for microplate liquid analysis.
  • To assess the system's capability for high-resolution, noncontact, and nondestructive measurement of fill height and volume.
  • To compare the system's performance against established methods.

Main Methods:

  • Utilized a Meniscense prototype system employing low-coherence interferometry.
  • The system captures both liquid fill height and meniscus shape.
  • Volume is calculated by combining fill height and meniscus data.
  • Gravimetric verification was performed using distilled water aliquots.

Main Results:

  • Achieved a liquid fill height resolution of 0.7 µm.
  • Demonstrated a volume resolution of 0.02 µL in a 96-well plate.
  • Gravimetric verification showed volume measurement inaccuracy of <2% for water.
  • The system handles various liquid types and meniscus shapes.

Conclusions:

  • Low-coherence interferometry provides a viable, high-resolution method for microplate liquid analysis.
  • The Meniscense system offers accuracy comparable to ratiometric photometry.
  • Its fill height resolution surpasses that of ultrasonic ranging, enhancing noncontact measurement capabilities.