Micellar Electrokinetic Chromatography of Aminoglycosides
Ulrike Holzgrabe1, Stefanie Schmitt2, Frank Wienen2
1Institut für Pharmazie und Lebensmittelchemie, Universität Würzburg, Am Hubland, Würzburg, 97074, Germany. u.holzgrabe@pharmazie.uni-wuerzburg.de.
Abstract:
The components of the aminoglycosides, e.g., gentamicin, sisomicin, netilmicin, kanamycin, amikacin, and tobramycin, and related impurities of these antibiotics can be separated by means of micellar electrokinetic chromatography (MEKC). Derivatization with o-phthaldialdehyde and thioglycolic acid is found to be appropriate for these antibiotics. The background electrolyte was composed of sodium tetraborate (100 mM), sodium deoxycholate (20 mM), and β-cyclodextrin (15 mM) having a pH value of 10.0. This method is valid for evaluation of gentamicin, kanamycin, and tobramycin. It has to be adopted for amikacin, paromomycin, neomycin, and netilmicin.
More Related Videos
10:41Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
Published on: April 5, 2019
11:44Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
Published on: January 19, 2022
Related Concept Videos
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Ion-Exchange Chromatography
Estimation of k and VD of Aminoglycosides
Affinity Chromatography
Electrophoresis: Overview
There...
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
