Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multimodal characterization of variation in neuronal types in the mouse basal ganglia.

bioRxiv : the preprint server for biology·2026
Same author

Bandage-Type Autocatalytic PdCl<sub>2</sub>‑Containing Film as Visual Hydrogen Sensor for Noninvasive Monitoring of Mg-Alloy Biodegradation.

ACS applied optical materials·2026
Same author

Morphoelectric Diversity and Specialization of Neuronal Cell Types in the Primate Striatum.

bioRxiv : the preprint server for biology·2026
Same author

Fishing with sound: Insights into the plainfin midshipman's reproductive ecology from field acoustic playbacks.

JASA express letters·2026
Same author

Genoarchitecture and input-output organization of the mouse basal ganglia and thalamic parafascicular nucleus.

Nature neuroscience·2026
Same author

A consensus spinal cord cell type atlas across mouse, macaque, and human.

bioRxiv : the preprint server for biology·2026
Same journal

TGF-β signaling regulates flat epithelium formation in severely injured adult mouse utricle through epithelial-mesenchymal transition.

Hearing research·2026
Same journal

Membrane scaffolding in auditory hair cells - a molecular tightrope walk enables lateral wall stiffness and flexibility.

Hearing research·2026
Same journal

Speech-in-noise recognition during hearing protector use: Human performance and acoustic prediction.

Hearing research·2026
Same journal

Estimation of hair cell loss from audiograms.

Hearing research·2026
Same journal

Cochlear size variation in a large-scale international multicentre cohort.

Hearing research·2026
Same journal

Estimation of minor-to-moderate conductive hearing loss with distortion-product otoacoustic emissions in humans.

Hearing research·2026
See all related articles

Related Experiment Video

Updated: Mar 14, 2026

An Assay for Lateral Line Regeneration in Adult Zebrafish
09:38

An Assay for Lateral Line Regeneration in Adult Zebrafish

Published on: April 8, 2014

13.2K

Hearing sensitivity differs between zebrafish lines used in auditory research.

J David Monroe1, Dustin P Manning2, Phillip M Uribe3

  • 1Department of Biology, Western Kentucky University, Bowling Green, KY, 42101, USA.

Hearing Research
|September 21, 2016
PubMed
Summary
This summary is machine-generated.

Zebrafish auditory studies require careful strain selection. Transgenic zebrafish expressing fluorescent proteins show altered auditory sensitivity, indicating potential mechanotransduction defects and early auditory issues.

Keywords:
Fluorescent proteinHair cellHearingTransgenicZebrafish

More Related Videos

In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish
08:51

In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish

Published on: November 28, 2018

12.2K
Author Spotlight: High-Throughput Toxicity Screening Using Zebrafish Embryo Startle Response Assay
06:25

Author Spotlight: High-Throughput Toxicity Screening Using Zebrafish Embryo Startle Response Assay

Published on: January 12, 2024

2.3K

Related Experiment Videos

Last Updated: Mar 14, 2026

An Assay for Lateral Line Regeneration in Adult Zebrafish
09:38

An Assay for Lateral Line Regeneration in Adult Zebrafish

Published on: April 8, 2014

13.2K
In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish
08:51

In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish

Published on: November 28, 2018

12.2K
Author Spotlight: High-Throughput Toxicity Screening Using Zebrafish Embryo Startle Response Assay
06:25

Author Spotlight: High-Throughput Toxicity Screening Using Zebrafish Embryo Startle Response Assay

Published on: January 12, 2024

2.3K

Area of Science:

  • Neuroscience
  • Auditory research
  • Zebrafish models

Background:

  • Zebrafish are valuable models for auditory research.
  • Transgenic zebrafish lines expressing fluorescent proteins in hair cells are common.
  • The impact of transgene expression on auditory function is not well understood.

Purpose of the Study:

  • To investigate how different zebrafish transgenic and background strains influence auditory sensitivity.
  • To identify potential mechanisms underlying auditory defects in transgenic zebrafish.

Main Methods:

  • Recording auditory evoked potentials (AEPs) in adult zebrafish.
  • Assessing FM1-43 uptake in hair cells to evaluate mechanotransduction.
  • Measuring prepulse inhibition in larval zebrafish as a behavioral auditory measure.
  • Comparing auditory sensitivity across multiple transgenic and background strains.

Main Results:

  • Three transgenic lines expressing hair cell-specific green fluorescent protein (GFP) exhibited significantly higher auditory thresholds.
  • Reduced FM1-43 uptake in Brn3c:mGFP zebrafish suggests a mechanotransduction defect.
  • Auditory defects were observed early in larval Brn3c:mGFP zebrafish (reduced prepulse inhibition).
  • Significant differences in auditory sensitivity were found between different background strains.

Conclusions:

  • Transgene expression and background strain significantly impact zebrafish auditory phenotype.
  • Researchers must exercise caution when selecting zebrafish strains for auditory studies.
  • Mechanisms underlying auditory defects vary among different transgenic lines.