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Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction

Masamitsu N Asaka1, Atsushi Kawaguchi1,2, Yuri Sakai2

  • 1Department of Infection Biology, Faculty of Medicine and Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.

Scientific Reports
|September 21, 2016
PubMed

Insights

Polycomb Repressive Complex 2 (PRC2) facilitates influenza virus production by aiding viral ribonucleoprotein (vRNP) complex assembly and nuclear export. Depleting PRC2 causes vRNP nuclear buildup, hindering virus release.

Area of Science:

  • Molecular Biology
  • Virology
  • Epigenetics

Background:

  • Nuclear domain organization is vital for viral infections.
  • Influenza virus export involves viral ribonucleoprotein (vRNP) complexes, M1, and NS2 proteins via CRM1.
  • The precise nuclear regulation of progeny vRNPs remains poorly understood.

Purpose of the Study:

  • To investigate the role of Polycomb Repressive Complex 2 (PRC2) in influenza virus replication.
  • To elucidate the spatio-temporal regulation of vRNP nuclear export.

Main Methods:

  • Depletion of PRC2 complex components.
  • Analysis of vRNP localization within the nucleus.
  • Assessment of M1-vRNP complex formation.
  • Co-immunoprecipitation to study PRC2-M1 interactions.

Main Results:

  • PRC2 acts as a positive factor in influenza virus production.
  • PRC2 depletion leads to nuclear accumulation of vRNP.
  • PRC2 depletion reduces M1-vRNP complex formation.
  • PRC2 directly binds to the M1 protein, facilitating M1-vRNP interaction.

Conclusions:

  • PRC2 is essential for efficient influenza virus production.
  • PRC2 mediates the recruitment of progeny vRNPs to facultative heterochromatin.
  • PRC2 facilitates the assembly of vRNPs into the M1-containing export complex.

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