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Poly r(C) binding protein (PCBP) 1 is a negative regulator of thyroid carcinoma

Mingpeng Zhang1, Xin Wang1, Jin Tan1

  • 1Department of Geriatrics, Tianjin Medical University General Hospital, Tianjin Geriatrics Institute Tianjin 300052, China.

American Journal of Translational Research
|September 21, 2016
PubMed

Insights

Poly r(C) binding protein 1 (PCBP1) acts as a tumor suppressor in thyroid cancer. Its downregulation by miR-490-3p promotes cancer progression, but restoring PCBP1 inhibits tumor formation.

Area of Science:

  • Molecular Biology
  • Oncology
  • RNA Biology

Background:

  • Poly r(C) binding protein 1 (PCBP1), also known as heterogeneous ribonucleoprotein E1, is an RNA-binding protein involved in various biological processes.
  • PCBP1 functions as a tumor suppressor in several cancers by inhibiting the translation of proteins that induce epithelial-mesenchymal transition (EMT).
  • Loss of PCBP1 expression or its phosphorylation can de-repress EMT inducers, but its specific role in thyroid carcinoma was previously unknown.

Purpose of the Study:

  • To investigate the role of PCBP1 in thyroid carcinoma.
  • To elucidate the regulatory mechanisms of PCBP1 expression in thyroid cancer.
  • To assess the therapeutic potential of PCBP1 in thyroid carcinoma.

Main Methods:

  • Analysis of PCBP1 expression in thyroid carcinoma patients.
  • In vitro kinase assays to assess Akt2-mediated phosphorylation of PCBP1.
  • In situ analysis and 3'UTR-based reporter assays to identify microRNA regulation.
  • Xenograft assays in mice to evaluate the tumor-suppressive function of PCBP1.

Main Results:

  • PCBP1 expression is significantly downregulated in thyroid carcinoma.
  • PCBP1 can be phosphorylated by Akt2 kinase in thyroid carcinoma cells.
  • PCBP1 is a target of miR-490-3p, which is highly expressed in thyroid carcinoma.
  • Overexpression of PCBP1 in thyroid cancer cells completely attenuated tumor formation in vivo.

Conclusions:

  • PCBP1 acts as a tumor suppressor in thyroid carcinoma.
  • Downregulation of PCBP1 in thyroid cancer is mediated by high expression of miR-490-3p.
  • Restoring PCBP1 expression holds potential for thyroid carcinoma therapy.
Keywords:
BCPAPPCBP1Poly r(C) binding proteinmiR-490

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