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Single-step purification and h.p.l.c. analysis of glutathione transferase 8-8 in rat tissues
1Cancer Research Campaign Molecular Toxicology Group, Department of Biochemistry, University College and Middlesex School of Medicine, London, U.K.
The Biochemical Journal
|June 15, 1989
Abstract:
GSSG selectively elutes two GSH transferases from a mixture of rat GSH transferases bound to a GSH-agarose affinity matrix. One is a form of GSH transferase 1-1 and the other is shown to be GSH transferase 8-8. By using tissues that lack this form of GSH transferase 1-1 (e.g. lung), GSH transferase 8-8 may thus be purified from cytosol in a single step. Quantitative analysis of the tissue distribution of GSH transferase 8-8 was obtained by h.p.l.c.