Related Experiment Videos
Nanosecond fluorometry of the single tryptophan in cytochrome P-450e (P450IIB2)
P Anzenbacher1, J Hudecek, S Vajda
1Department of Biochemistry, Charles University, Prague, Czechoslovakia.
Biochemical and Biophysical Research Communications
|August 15, 1989
Abstract:
Properties of the single tryptophan residue in rat liver microsomal phenobarbital-inducible cytochrome P-450e (P450IIB2) were studied by the nanosecond time-resolved fluorometry. The tryptophan fluorescence decay time was found to be 3.6 ns and it was not affected by the addition of substrate (perhydrophenanthrene). This result strongly indicates that the tryptophan residue is not a part of the substrate-binding site.