Different binding of propranolol enantiomers to human alpha 1-acid glycoprotein

The binding of propranolol enantiomers to human alpha 1-acid glycoprotein was studied using high performance liquid chromatography in order to provide insight into binding models and to describe individual binding parameters of both enantiomers. The binding of (-)-propranolol was shown to be saturable with one major binding site (n = 0.81, k = 2.73 x 10(5)/M). The saturation process achieved its upper asymptotic value at drug/protein molar ratio of approximately 1. In the case of the opposite (+)-enantiomer the binding isotherm did not show evidence of saturation even at higher drug/protein molar ratios (up to 50). The individual binding parameters for (+)-enantiomer were n = 0.38, k = 3.4 x 10(6)/M and n'k' = 1.39 x 10(4)/M for the saturable and nonsaturable binding component, respectively. At drug/protein molar ratio 2 the circular dichroism measurements confirmed the existence of different binding models for individual propranolol enantiomers.