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Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
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Identifying and annotating human bifunctional RNAs reveals their versatile functions.

Geng Chen1, Juan Yang2, Jiwei Chen2

  • 1Center for Pharmacogenomics, School of Pharmacy and School of Life Sciences, Fudan University, Shanghai, 201203, China.

Science China. Life Sciences
|September 22, 2016
PubMed
Summary
This summary is machine-generated.

This study identifies 132 human bifunctional RNAs with both protein-coding and noncoding roles, revealing their involvement in development, gene regulation, and potential links to cancer mutations.

Keywords:
RNA-seqbifunctional RNAnoncoding RNAtandem mass spectrometry

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • Bifunctional RNAs, possessing both protein-coding and noncoding functions, remain under-explored and poorly understood.
  • Their complex roles in cellular processes and disease pathogenesis require systematic investigation.

Purpose of the Study:

  • To systematically characterize the features and functions of human bifunctional RNAs.
  • To develop a reliable pipeline for identifying and annotating these unique RNA molecules.
  • To elucidate their involvement in gene regulation, development, and disease.

Main Methods:

  • Integration of tandem mass spectrometry and RNA-sequencing data.
  • Development of a computational pipeline for bifunctional RNA identification and annotation.
  • Bioinformatic analyses to predict interactions with miRNAs and RNA-binding proteins.

Main Results:

  • Characterization of 132 high-confidence human bifunctional RNAs.
  • Evidence suggesting bifunctional RNA involvement in embryonic development and broad tissue functionality.
  • Identification of interactions with multiple miRNAs and RNA-binding proteins.
  • Demonstration of bifunctional RNAs acting as competing endogenous RNAs (ceRNAs) to regulate gene expression.
  • Association of somatic mutations in carcinomas with potential detrimental effects on bifunctional RNAs.

Conclusions:

  • The developed pipeline enables robust identification and annotation of bifunctional RNAs.
  • Bifunctional RNAs play significant gene-regulatory roles through complex interactions.
  • These RNAs are implicated in human development and disease, including cancer.