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The Protective Effect of Poloxamer-188 on Platelet Functions
Nil Guler1, Schuharazad Abro1, Marty Emanuele2
11 Department of Pathology and Pharmacology, Loyola University Medical Center, Maywood, IL, USA.
Background:
Poloxamer-188 (MST-188) is effective in the repair/recovery of damaged cell membranes. MST-188 is a promising agent for protecting blood cell viability. The aim of the study is to test the hypothesis that MST-188 can extend the duration of platelet function.
Materials And Methods:
Blood samples were collected from 20 healthy volunteers. MST-188 (10 or 2 mg/mL) containing platelet-rich plasma (PRP) was prepared with 2 procedures. First, PRP prepared from MST-188 added whole blood (WB); second, MST-188 was added to PRP. These were referred to MST-188-WB preparation (WBP) and MST-188-PRP preparation (PRPP), respectively. For control, saline was used in the same manner. Agonist-induced aggregation (AIA) studies were performed at 30, 180, and 300 minutes using Platelet Aggregation Profiler (PAP-8) aggregometer (Bio/Data Corporation, Horsham, Pennsylvania) and Adenosine diphosphate (ADP), arachidonic acid, collagen, and epinephrine as agonists at final concentration of 20 µM, 500 µg/mL, 0.19 mg/mL, and 100 µM, respectively.
Results:
There was a protective effect of MST-188 on ADP and collagen AIA. At 300 minutes, ADP AIA was found to be 50.2% higher than saline control in 2-mg WBP, 43% at 10-mg PRPP, and 10.4% at 2-mg PRPP. Protective effect of on collagen AIA was 65.9% in 2-mg WBP, 42.74% at 10-mg PRPP, and 11.42% at 2-mg PRPP. In comparison between 30 and 300 minutes, MST-188 showed significant protection in terms of ADP and collagen receptors and for both types of preparations (WBP and PRPP).
Conclusion:
The protective effects of MST-188 on ADP- and collagen-induced platelet aggregation may contribute to the preservation of platelet functionality upon storage in blood banks.
Insights
Poloxamer-188 (MST-188) effectively preserves platelet function by protecting against ADP- and collagen-induced aggregation. This finding suggests MST-188 can enhance platelet viability during storage.
Area of Science:
- Biomedical Science
- Hematology
- Cell Biology
Background:
- Poloxamer-188 (MST-188) demonstrates efficacy in repairing damaged cell membranes and protecting blood cell viability.
- MST-188 is investigated for its potential to extend the functional lifespan of platelets.
Purpose of the Study:
- To evaluate the hypothesis that MST-188 can prolong platelet function duration.
- To assess the protective effects of MST-188 on platelet aggregation.
Main Methods:
- Blood samples from 20 healthy volunteers were used to prepare platelet-rich plasma (PRP).
- MST-188 was added to whole blood (WBP) or PRP (PRPP) at 2 or 10 mg/mL concentrations.
- Agonist-induced aggregation (AIA) was measured at 30, 180, and 300 minutes using Adenosine diphosphate (ADP), collagen, and other agonists.
Main Results:
- MST-188 exhibited a protective effect on ADP- and collagen-induced platelet aggregation.
- At 300 minutes, ADP AIA was significantly higher with MST-188 compared to saline controls.
- Significant protection was observed for both ADP and collagen aggregation across WBP and PRPP preparations over time.
Conclusions:
- MST-188 demonstrates protective effects against ADP- and collagen-induced platelet aggregation.
- These findings suggest MST-188 can contribute to preserving platelet functionality during storage in blood banks.
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