Related Experiment Video
Updated: Mar 14, 2026
Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
Published on: July 13, 2017
Identification and tissue expression profiling of odorant binding protein genes in the red palm weevil, Rhynchophorus
Wei Yan1, Li Liu2, Weiquan Qin2
1Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing, 100083 China ; Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan, 571339 China.
Background:
The red palm weevil, Rhynchophorus ferrugineus, is a lethal pest of the palms. The identification of odorant binding protein (OBP) genes will be helpful for clarifing the mechanism of odorant detection of this pest. By sequencing the full length cDNA library of its antenne, 11 OBP genes (RferOBP1-11) were identified.
Findings:
The result showed RferOBP1-7 and RferOBP8-11 belonged to the minus-C and classic family, respectively qPCR revealed that RferOBP1-10 highly transcribed in the antennae, of which RferOBP1, RferOBP4, RferOBP8 and RferOBP10 were obviously male-biased expression. RferOBP7 and RferOBP11 exhibited highly expression in female head and male thorax. RferOBP2, RferOBP5 and RferOBP6 were highly expressed in the female thorax, leg and abdomen respectively.
Conclusions:
The results paved the way towards a future understanding of the olfaction in this species.
