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Updated: Mar 14, 2026
A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms
Published on: September 16, 2022
Candida tropicalis biofilm inhibition by ZnO nanoparticles and EDTA
Vinoth Jothiprakasam1, Murugan Sambantham1, Stalin Chinnathambi1
1CAS in Marine Biology, Annamalai University, Parangipettai-608 502, Tamil Nadu, India.
Objective:
Biofilm of Candida tropicalis denote as a complex cellular congregation with major implication in pathogenesis. This lifestyle of fungus as a biofilm can inhibit immune system and antifungal therapy in treatment of infectious disease especially medical device associated chronic disease. In this study effects of Zinc Oxide (ZnO) nanoparticles and EDTA were evaluated on C. tropicalis biofilm by using different techniques. ZnO nanoparticles were synthesized from Egg albumin.
Design:
To assay the formation of biofilm of yeast cells like Fluconazole-susceptible C. tropicalis (ATCC 13,803) and fluconazole-resistant standard strains of C. tropicalis (ATCC 750) were grown in 24 well plates and antifungal effect of ZnO and EDTA were evaluated on C. tropicalis biofilm using ATP bioluminescence and tetrasodium salt (XTT) reduction assays.
Results:
Synthesized ZnO NPs and EDTA had effective antifungal properties at the concentration of 5.2, 8.6μg/ml for Fluconazole susceptible strain and 5.42, 10.8μg/ml Fluconazole resistant strains of C. tropicalis biofilms compared to fluconazole drug.
Conclusion:
In present study we conclude, ZnO considered as a new agent in field of prevention C. tropicalis biofilms especially biofilms formed surface of medical device.
Insights
Zinc Oxide (ZnO) nanoparticles and EDTA effectively inhibit Candida tropicalis biofilms, offering a new strategy for preventing medical device-associated infections. These agents show promise against both fluconazole-susceptible and resistant strains.
Area of Science:
- Microbiology
- Nanotechnology
- Biomedical Engineering
Background:
- Candida tropicalis biofilms pose significant challenges in treating chronic and medical device-associated infections.
- Biofilm formation by C. tropicalis can lead to immune evasion and resistance to antifungal therapies.
Purpose of the Study:
- To evaluate the efficacy of Zinc Oxide (ZnO) nanoparticles and EDTA against Candida tropicalis biofilms.
- To explore ZnO nanoparticles synthesized from egg albumin as a potential anti-biofilm agent.
Main Methods:
- Culturing of fluconazole-susceptible and resistant C. tropicalis strains.
- Assessing biofilm formation and antifungal effects using ATP bioluminescence and XTT reduction assays.
- Synthesis of ZnO nanoparticles using egg albumin.
Main Results:
- Both synthesized ZnO nanoparticles and EDTA demonstrated significant antifungal properties against C. tropicalis biofilms.
- Effective concentrations varied for susceptible and resistant strains, showing comparable or superior efficacy to fluconazole.
Conclusions:
- ZnO nanoparticles represent a promising new agent for preventing C. tropicalis biofilms.
- These findings highlight the potential of ZnO in combating biofilm formation on medical devices.
