Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Theoretical Study on the Quantum Dynamics of Highly Energetic <sup>14</sup>N + <sup>15</sup>N<sup>15</sup>N Isotope Exchange Reactions.

The journal of physical chemistry. A·2026
Same author

Editorial: Neuromuscular disorders: biomarkers, precision diagnosis, and targeted therapeutics.

Frontiers in neuroscience·2026
Same author

Nanostructured implants for enhanced integration and localized therapy.

BioImpacts : BI·2026
Same author

Investigating the Photodetachment Spectrum of Al <math><semantics><mrow><msub><mi></mi> <mn>3</mn></msub></mrow> <annotation>$_3$</annotation></semantics></math> O <math><semantics><mrow><msubsup><mi></mi> <mn>3</mn> <mo>-</mo></msubsup></mrow> <annotation>$_3^-$</annotation></semantics></math> : A Theoretical Approach.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same author

Hypertension knowledge gaps: A patient-caregiver comparison from a tertiary care centre in Northern India.

Heart international·2026
Same author

Postnatal Care Utilisation Among Adolescent Mothers in India: A Pooled Cross-Sectional Study of NFHS-4 and NFHS-5.

Public health challenges·2026

Related Experiment Video

Updated: Mar 14, 2026

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
12:51

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

Published on: November 29, 2012

17.3K

Saturation Dynamics Measures Absolute Cross Section and Generates Contrast within a Neuron.

Suraj Kumar1, Aditya Singh1, Vijay R Singh2

  • 1Center for Neuroscience, Indian Institute of Science, Bangalore, Karnataka, India.

Biophysical Journal
|September 23, 2016
PubMed
Summary

We developed a new method to measure the multiphoton cross section of fluorophores, revealing tryptophan

More Related Videos

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
08:48

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Published on: September 5, 2012

12.4K
Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice
07:04

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Published on: January 7, 2019

11.9K

Related Experiment Videos

Last Updated: Mar 14, 2026

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
12:51

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

Published on: November 29, 2012

17.3K
Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
08:48

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Published on: September 5, 2012

12.4K
Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice
07:04

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Published on: January 7, 2019

11.9K

Area of Science:

  • Photophysics
  • Biophysics
  • Spectroscopy

Background:

  • Optical saturation of chromophores depends on absorption cross-section and excited-state lifetime.
  • Understanding these properties is crucial for quantitative fluorescence microscopy.

Purpose of the Study:

  • To present an analytical description of optical saturation.
  • To develop a generalized method for measuring multiphoton cross-sections.
  • To introduce a new contrast parameter (β) for imaging local environments.

Main Methods:

  • Analytical modeling of chromophore saturation.
  • Measurement of absolute three-photon cross-section spectra.
  • Intensity scans in a two-photon microscope setup for β-imaging.

Main Results:

  • Fluorescence at saturation onset is characterized by the product of absorption cross-section and lifetime.
  • Absolute three-photon cross-section spectra of tryptophan were obtained (0.28–20 S.I. units).
  • β-imaging revealed concentration-independent contrast variations in EGFP-labeled mouse brain slices.

Conclusions:

  • The product of absorption cross-section and lifetime is a key parameter in saturation studies.
  • Multiphoton cross-section measurements provide valuable photophysical data.
  • β-imaging offers a novel, concentration-independent contrast mechanism for microscopy.