Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Mar 14, 2026

Dissection of Larval Zebrafish Gonadal Tissue

Dissection of Larval Zebrafish Gonadal Tissue

Published on: April 26, 2017

See-Thru-Gonad zebrafish line: developmental and functional validation.

Christopher Presslauer¹, Teshome Tilahun Bizuayehu¹, Komeil Razmi¹

¹Faculty of Biosciences and AquacultureNord University, Bodø, Norway.

Reproduction (Cambridge, England)

|September 23, 2016

Summary

We developed See-Thru-Gonad zebrafish, a transparent line for visualizing germ cells throughout life. This model enables studying gene knockdown effects, like miR-92a-3p, transmitted from parent ovaries to offspring.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Primordial Germ Cell Development in Teleosts: Their Origin, Fate and Role in Sex Determination.

Stem cell reviews and reports·2026

Same author

Integrated Sensory and Immune Cell Organization in the Lip Skin of the Goldfish (<i>Carassius auratus</i>, Linnaeus, 1758).

Animals : an open access journal from MDPI·2026

Same author

Deep breath out: molecular survey of selected pathogens in blow and skin biopsies from North Atlantic cetaceans.

BMC veterinary research·2025

Same author

Early Nutrition Impacts on Growth, Skeletal Anomalies and Organ Ontogeny in Larval Atlantic Cod (<i>Gadus morhua</i>).

Animals : an open access journal from MDPI·2025

Same author

Decitabine-induced DNA methylation remodeling reveals targetable biological processes in gilthead seabream pituitary and liver explants.

Epigenetics·2025

Same author

Optimization of Nile Tilapia Artificial Breeding Using Human Chorionic Gonadotropin (hCG) Hormone.

Methods and protocols·2025

Area of Science:

Reproductive biology
Developmental biology
Zebrafish models

Background:

Zebrafish are crucial for developmental biology research.
Their utility in reproductive biology remains underexplored.
A transparent zebrafish model with visible germ cells is needed.

Purpose of the Study:

Establish a transparent zebrafish line (See-Thru-Gonad) with fluorescently labeled germ cells.
Validate gonadal development and germ cell visibility across the life cycle.
Demonstrate the line's utility for studying gene function in reproduction via targeted knockdown.

Main Methods:

Crossed specific zebrafish lines (zf45Tg and mitfa(w2/w2); mpv17(b18/b18)) to create the See-Thru-Gonad line.
Documented in vivo germline fluorescence from gametes to sexual maturity.

More Related Videos

Author Spotlight: Enhanced Method for Isolating Pure Zebrafish Stage I Oocytes

Author Spotlight: Enhanced Method for Isolating Pure Zebrafish Stage I Oocytes

Published on: July 26, 2024

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

Published on: November 30, 2022

Related Experiment Videos

Last Updated: Mar 14, 2026

Dissection of Larval Zebrafish Gonadal Tissue

Dissection of Larval Zebrafish Gonadal Tissue

Published on: April 26, 2017

Author Spotlight: Enhanced Method for Isolating Pure Zebrafish Stage I Oocytes

Author Spotlight: Enhanced Method for Isolating Pure Zebrafish Stage I Oocytes

Published on: July 26, 2024

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

Published on: November 30, 2022

Validated gonadal development using histology.

Performed targeted gene knockdown of miR-92a-3p using vivo-morpholinos (VMOs) injected into ovaries.

Assessed offspring for developmental effects and miR-92a-3p expression.

Main Results:

The See-Thru-Gonad line exhibits clear, life-long germ cell fluorescence.
Histology confirmed normal gonadal development.
miR-92a-3p knockdown in ovaries led to reduced miRNA expression in offspring.
Offspring from knockdown experiments showed increased developmental arrest at the 1-cell stage.

Conclusions:

The See-Thru-Gonad zebrafish line provides unprecedented visibility of germ cells throughout development.
This model is effective for studying the functional impact of ovarian gene knockdown.
The study demonstrates vertical transmission of gene knockdown effects from parent to offspring, validating the model's application in reproductive biology research.