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The Nematode Caenorhabditis Elegans - A Versatile In Vivo Model to Study Host-microbe Interactions

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Probiotic Enterococcus faecalis Symbioflor^® down regulates virulence genes of EHEC in vitro and decrease

Klaus Neuhaus¹, Marina C Lamparter^2,3, Benjamin Zölch¹

¹Lehrstuhl für Mikrobielle Ökologie, Wissenschaftszentrum Weihenstephan, Technische Universität München, Weihenstphaner Berg 3, 85350, Freising, Germany.

Archives of Microbiology

|September 23, 2016

Summary

This summary is machine-generated.

The probiotic Enterococcus faecalis Symbioflor® protects Caenorhabditis elegans from enterohemorrhagic E. coli O157:H7 (EHEC) by altering bacterial gene expression. This probiotic enhances worm lifespan by downregulating EHEC virulence factors.

Keywords:

Caenorhabditis elegans EHEC Enterococcus faecalis Symbioflor®Transcriptome Virulence lux transposons

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Published on: December 3, 2019

Area of Science:

Microbiology
Genomics
Nematology

Background:

Enterohemorrhagic E. coli O157:H7 (EHEC) infections pose significant health risks.
Caenorhabditis elegans serves as a model organism for studying host-pathogen interactions and aging.
Probiotics are being investigated for their potential to mitigate bacterial infections.

Purpose of the Study:

To investigate the protective effect of Enterococcus faecalis Symbioflor® against EHEC in C. elegans.
To elucidate the transcriptomic changes in EHEC induced by Symbioflor®.
To determine the role of specific EHEC genes in the observed protective effect.

Main Methods:

Co-feeding EHEC with Symbioflor® to C. elegans.
RNA sequencing (RNA-seq) to analyze EHEC transcriptome.
Construction and analysis of lux-transposon mutants and gene knockouts in EHEC.
Complementation assays to verify gene function.

Main Results:

Symbioflor® significantly increased C. elegans lifespan during EHEC infection.
RNA-seq revealed downregulation of key EHEC virulence genes (e.g., LEE, flagellum, quorum-sensing) in the presence of Symbioflor®.
Upregulation of acid response and cellular stress genes in EHEC was observed, alongside evidence of prophage activity and cell lysis.
Knockout of specific virulence genes (fliD, espB, Z3136, Z3917, L7052) altered nematode lifespan, confirming their role in EHEC pathogenesis and Symbioflor®'s modulation.

Conclusions:

Enterococcus faecalis Symbioflor® confers protection to C. elegans against EHEC infection.
The protective mechanism involves Symbioflor®-induced transcriptomic alterations in EHEC, including downregulation of virulence and upregulation of stress responses.
Symbioflor® shows potential as a protective probiotic agent against EHEC in a nematode model.