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Data on the mechanisms underlying succinate-induced aortic contraction.

Natália A Gonzaga1, Janaina A Simplicio1, Letícia N Leite1

  • 1Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (USP), Ribeirão Preto, SP, Brazil; Laboratório de Farmacologia, DEPCH, Escola de Enfermagem de Ribeirão Preto, USP, Ribeirão Preto, SP, Brazil.

Data in Brief
|September 23, 2016
PubMed
Summary

Succinate causes vascular contraction by activating the GPR91 receptor. This process involves p38 MAPK, JNK, Rho-kinase, and protein kinase C signaling pathways, leading to vasoconstriction.

Keywords:
AortaContractionSuccinate

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Area of Science:

  • Pharmacology
  • Physiology
  • Molecular Biology

Background:

  • Succinate is a metabolic intermediate with signaling functions.
  • G-protein-coupled receptor 91 (GPR91) is a known receptor for succinate.
  • Vascular effects of succinate require further elucidation.

Purpose of the Study:

  • To investigate the mechanisms of succinate-induced vascular contraction.
  • To determine the role of GPR91 in succinate's vascular effects.
  • To identify the signaling pathways involved in succinate-mediated vasoconstriction.

Main Methods:

  • Vascular contraction assays using isolated rat and mouse aortic rings.
  • Experiments utilizing GPR91-deficient mice.
  • Pharmacological inhibition of specific signaling pathways (p38 MAPK, JNK, Rho-kinase, ERK1/2, PKC, COX).
  • Assessment of calcium channel blockade and superoxide anion scavenging.

Main Results:

  • Succinate induced contraction in endothelium-intact and denuded aortic rings.
  • Succinate's effect was diminished in GPR91-deficient mice.
  • Inhibitors of p38 MAPK, JNK, and Rho-kinase reduced succinate-induced contraction.
  • Indomethacin, H7, verapamil, and tiron attenuated the contractile response.

Conclusions:

  • Succinate mediates vascular contraction, partly through GPR91 activation.
  • The contraction involves p38 MAPK, JNK, Rho-kinase, protein kinase C, cyclooxygenase, calcium influx, and superoxide anion production.
  • These findings highlight succinate's role as a vasoactive signaling molecule.