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Updated: Mar 14, 2026

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
Published on: August 20, 2018
The Pil3 pilus of Streptococcus gallolyticus binds to intestinal mucins and to fibrinogen
Mariana Martins1,2, Constance Porrini1,2, Laurence du Merle1,2
1a Department of Microbiology , Biology of Gram-positive Pathogens Unit, Institut Pasteur , Paris , France.
Abstract:
Streptococcus gallolyticus is a commensal bacterium responsible for infectious endocarditis in the elderly, which has frequently been associated with colonic carcinoma. Whether this species is a cause or a consequence of colorectal cancer remains unknown. We recently demonstrated that S. gallolyticus Pil3 pilus is required for adhesion to colonic mucus and for colonization of mouse distal colon. We show here that Pil3 pilus binds equally well to human colonic mucins derived from HT29-MTX cells and to human stomach mucins from healthy donors. In addition, we have found that Pil3 also binds to human fibrinogen, which expands the repertoire of Pil3 host ligands.
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