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Published on: August 9, 2019
Peripheral and bone marrow CD34+ cell levels on chronic myeloproliferative disease
M Pamukcuoglu1, K Acar2, B Celik3
1a Department of Hematology , Ankara Numune Education and Research Hospital , Ankara , Turkey.
Insights
Peripheral and bone marrow CD34+ cell levels in myeloproliferative disease (MPD) patients showed no correlation with most clinicopathologic characteristics. However, peripheral CD34+ cells correlated with bone marrow fibrosis, and bone marrow CD34+ cells correlated with constitutional symptoms.
Area of Science:
- Hematology
- Oncology
- Stem Cell Biology
Background:
- Myeloproliferative neoplasms (MPNs) are a group of clonal hematopoietic stem cell disorders.
- CD34+ cells, including hematopoietic stem and progenitor cells (HSPCs), play a crucial role in MPN pathogenesis.
- Understanding the relationship between CD34+ cell levels and disease characteristics is important for patient management.
Purpose of the Study:
- To investigate the association between peripheral and bone marrow CD34+ cell counts and various clinicopathologic and laboratory parameters in patients with myeloproliferative diseases (MPDs).
Main Methods:
- A cohort of 103 MPD patients was analyzed.
- Peripheral blood and bone marrow samples were collected to quantify CD34+ cell levels.
- Statistical analyses were performed to correlate CD34+ cell counts with clinical and laboratory findings, including JAK-2 V617F mutation, thrombosis, white blood cell count, lactate dehydrogenase, transferrin saturation, ferritin, bone marrow cellularity, bone marrow fibrosis, and constitutional symptoms.
Main Results:
- No significant correlations were observed between peripheral CD34+ levels and JAK-2 V617F mutation, thrombosis, white blood cells, lactate dehydrogenase, transferrin saturation, ferritin, or bone marrow cellularity.
- Similarly, bone marrow CD34+ levels did not significantly correlate with these parameters.
- Significant positive correlations were found between peripheral CD34+ levels and bone marrow fibrosis (P < 0.001), and between bone marrow CD34+ levels and constitutional symptoms (P < 0.05) and bone marrow fibrosis (P < 0.001).
Conclusions:
- The study found no significant relationship between peripheral or bone marrow CD34+ cell counts and most clinicopathologic and laboratory characteristics in MPD patients, except for bone marrow fibrosis and constitutional symptoms.
- The lack of correlation between peripheral and bone marrow CD34+ levels suggests that peripheral CD34+ cells may originate from extramedullary sites, such as the spleen, rather than solely from the bone marrow.
Purpose:
The aim of the present study was to examine the relationship between peripheral CD34+ and bone marrow CD34+ levels and the clinicopathologic characteristics and laboratory parameters of myeloproliferative disease (MPD) patients.
Patients And Methods:
A total of 103 MPD patients were enrolled in this study. We examined the relationship between bone marrow CD34+ and peripheral CD34+ levels and the patients' clinicopathologic and laboratory parameters.
Results:
There were no significant correlations between the peripheral CD34+ levels and the JAK-2 V617F mutation, thrombosis, white blood cells (WBC), lactate dehydrogenase (LDH), transferrin saturation (TS), ferritin, or bone marrow cellularity. In addition, there were no significant correlations between bone marrow CD34+ levels and the JAK-2 V617F mutation, thrombosis, WBC, LDH, TS, ferritin, or bone marrow cellularity (P > 0.05). We did not identify any significant relationship between peripheral CD34+ and bone marrow CD34+ levels (P > 0.05). However, there were significant correlations between peripheral CD34+ levels and bone marrow fibrosis (P < 0.001), between bone marrow CD34+ levels and constitutional symptoms (P < 0.05), and between bone marrow CD34+ levels and bone marrow fibrosis (P < 0.001).
Conclusion:
We did not find any significant relationship between the clinicopathologic and laboratory characteristics and peripheral and bone marrow CD34+ cells from bone marrow fibrosis patients. There was also no significant relationship between bone marrow CD34+ cells and peripheral CD34+ cells. Some peripheral CD34+ cells may originate from the spleen rather than the bone marrow, which may given us different result of some parameters.
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