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Related Experiment Videos

A modified horizontal capillary puller for fabrication of patch-clamp pipettes.

G A Mealing1, J L Schwartz

  • 1Division of Biological Sciences, National Research Council, Ottawa, Ontario, Canada.

Brain Research Bulletin
|May 1, 1989
PubMed
Summary

A simple modification enables a conventional micropipette puller to create specialized pipettes for patch-clamp studies. This method bypasses the hard-pull stage and uses two distinct heating cycles.

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Area of Science:

  • Biophysics
  • Neuroscience
  • Cell Biology

Background:

  • Patch-clamp electrophysiology is a critical technique for studying ion channels and cellular electrical activity.
  • Conventional micropipette pullers often require expensive modifications or specialized equipment to produce high-quality patch pipettes.
  • The fabrication of precise micropipettes is essential for successful and reproducible patch-clamp recordings.

Purpose of the Study:

  • To describe a simple, low-cost modification for a conventional horizontal two-stage micropipette puller.
  • To enable the production of high-quality pipettes suitable for patch-clamp studies using modified equipment.
  • To provide a cost-effective solution for researchers needing patch pipettes.

Main Methods:

  • Modification of a conventional horizontal two-stage micropipette puller (Industrial Science Associates, Inc. M-1).

Related Experiment Videos

  • Disabling the hard-pull stage of the fabrication process.
  • Implementing two distinct heating cycles with varied filament temperatures during pipette pulling.

    • Main Results:

    • Successful production of pipettes suitable for patch-clamp studies using the modified puller.
    • The modification is straightforward and low-cost.
    • The method is adaptable to other horizontal two-stage pullers utilizing a solenoid for pull force.

    Conclusions:

    • A simple, cost-effective modification can adapt standard micropipette pullers for patch-clamp pipette fabrication.
    • This technique offers an accessible alternative for researchers requiring specialized patch pipettes.
    • The described method enhances the utility of conventional micropipette pullers in biological research.