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A Rapid TALEN Assembly Protocol.

Arslan Akmammedov1, Tomonori Katsuyama2, Renato Paro3,4

  • 1Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule Zürich, Mattenstrasse 26, 4058, Basel, Switzerland. arslan.akmammedov@bsse.ethz.ch.

Methods in Molecular Biology (Clifton, N.J.)
|September 24, 2016
PubMed
Summary
This summary is machine-generated.

Transcription activator-like effector nucleases (TALENs) enable precise DNA editing. This study presents easyT for TALEN assembly and a homologous recombination strategy for efficient genome engineering.

Keywords:
Genome engineeringSite-specific gene integrationTALEN

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Transcription activator-like effector nucleases (TALENs) offer modular DNA recognition.
  • TALENs are widely used for creating site-specific DNA double-strand breaks (DSBs).

Purpose of the Study:

  • To describe an efficient method for TALEN assembly named easyT.
  • To present a strategy for genome engineering utilizing homologous recombination (HR).

Main Methods:

  • TALEN assembly using the easyT method.
  • Genome engineering employing homologous recombination (HR) with donor DNA.

Main Results:

  • The easyT method facilitates TALEN construction.
  • The described HR strategy enables precise sequence alterations in the genome.

Conclusions:

  • The easyT method simplifies TALEN-based genome engineering.
  • This approach enhances the precision of genetic modifications via homologous recombination.