Related Experiment Video
Updated: Aug 21, 2026

Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field
Published on: August 2, 2011
Complete Genome Sequence of the Avian Paramyxovirus Serotype 5 Strain APMV-5/budgerigar/Japan/TI/75
Takahiro Hiono1, Keita Matsuno2, Kotaro Tuchiya3
1Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, Japan.
Abstract:
Here, we report the complete genome sequence of the avian paramyxovirus serotype 5 strain APMV-5/budgerigar/Japan/TI/75, which was determined using the Illumina MiSeq platform. The determined sequence shares 97% homology and similar genetic features with the previously known genome sequence of avian paramyxovirus serotype 5 strain APMV-5/budgerigar/Japan/Kunitachi/74.
Insights
The complete genome sequence of avian paramyxovirus serotype 5 strain APMV-5/budgerigar/Japan/TI/75 was determined. This new avian paramyxovirus 5 sequence shows high homology to a previously identified strain.
Area of Science:
- Virology
- Genomics
Background:
- Avian paramyxoviruses (APMV) are significant pathogens in poultry.
- APMV serotype 5 (APMV-5) is a specific subtype with implications for avian health.
Purpose of the Study:
- To determine and report the complete genome sequence of a novel APMV-5 strain.
- To compare the genetic features of this new strain with existing APMV-5 sequences.
Main Methods:
- Whole-genome sequencing using the Illumina MiSeq platform.
- Bioinformatic analysis for sequence determination and comparison.
Main Results:
- The complete genome sequence for APMV-5/budgerigar/Japan/TI/75 was successfully obtained.
- The determined sequence exhibited 97% homology with APMV-5/budgerigar/Japan/Kunitachi/74.
- Genetic features were found to be similar between the two APMV-5 strains.
Conclusions:
- The genomic data provides valuable information on APMV-5 diversity.
- This finding contributes to understanding the genetic relatedness of APMV-5 strains in Japan.
More Related Videos
12:09Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved
Published on: August 2, 2011
07:15Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity
Published on: November 22, 2021